-transparent.png){kind=logo}
The synthesis and deposition of 22,000-dalton (22K) spore coat protein were examined immunochemically on the sporulating cells of Bacillus megaterium ATCC 12872 using the antibody to purified 22K spore coat protein. This antibody cross-reacted with 44K and 25K proteins in immunoblot analysis of dormant spore coat proteins. Immunoblot analysis on the sporulating cells showed that 22K protein was detected from t8 in forespore coat protein fractions. Sandwich enzyme immunoassay revealed that 22K protein in the spore coat protein fraction appeared at t6 and reached a plateau at t9, and 22K protein in the mother cell cytoplasmic fraction was detected at only t7 and t8 at a very low level.
T Nishihara, and
K Sakagami, and
K Tani, and
M Imagawa, and
M Kondo
January 1987,
Microbiology and immunology,
T Nishihara, and
K Sakagami, and
K Tani, and
M Imagawa, and
M Kondo
January 1972,
Zeitschrift fur allgemeine Mikrobiologie,
T Nishihara, and
K Sakagami, and
K Tani, and
M Imagawa, and
M Kondo
October 1966,
The Journal of biological chemistry,
T Nishihara, and
K Sakagami, and
K Tani, and
M Imagawa, and
M Kondo
July 1977,
Biochimica et biophysica acta,
T Nishihara, and
K Sakagami, and
K Tani, and
M Imagawa, and
M Kondo
June 1971,
The Journal of biological chemistry,
T Nishihara, and
K Sakagami, and
K Tani, and
M Imagawa, and
M Kondo
May 1975,
Journal of bacteriology,
T Nishihara, and
K Sakagami, and
K Tani, and
M Imagawa, and
M Kondo
January 1978,
Microbios,
T Nishihara, and
K Sakagami, and
K Tani, and
M Imagawa, and
M Kondo
May 1973,
Biochemical and biophysical research communications,
T Nishihara, and
K Sakagami, and
K Tani, and
M Imagawa, and
M Kondo
September 1980,
Journal of general microbiology,
T Nishihara, and
K Sakagami, and
K Tani, and
M Imagawa, and
M Kondo
November 1996,
Applied and environmental microbiology,
