-transparent.png){kind=logo}
A new method of detecting the C3b receptor is reported. A particular merit of this method is that anti-RBC rabbit antiserum is not required. Rosettes were formed with human B lymphocytes, B lymphoblasts and granulocytes, using sheep erythrocytes (SRBC) sensitized with fresh human serum (FHS). T lymphocytes and T lymphoblasts did not form rosettes. The percentage of cells forming rosettes with this method approximated the percentage of rosettes formed with EACm. However, FHS coated SRBC did not react with most cells of B cell type chronic lymphocytic leukemia (CLL), whereas EACm rosette formations showed a definite reaction. On the other hand, 34--58% of cells of chronic myelocytic leukemia (CML) bound with the indicator red cells. SRBC sensitized with fresh rabbit or guinea pig serum formed rosettes with PBL, tonsil cells, B lymphoblasts and granulocytes. Complement and IgM antibody were required for this reaction, as in EAC rosette formation.
P M Chen, and
N Yamanaka, and
K Kikuchi, and
K Kitajima, and
I Kimura
August 1974,
Journal of immunology (Baltimore, Md. : 1950),
P M Chen, and
N Yamanaka, and
K Kikuchi, and
K Kitajima, and
I Kimura
January 1972,
Lancet (London, England),
P M Chen, and
N Yamanaka, and
K Kikuchi, and
K Kitajima, and
I Kimura
April 1976,
The Journal of clinical investigation,
P M Chen, and
N Yamanaka, and
K Kikuchi, and
K Kitajima, and
I Kimura
October 1974,
The New England journal of medicine,
P M Chen, and
N Yamanaka, and
K Kikuchi, and
K Kitajima, and
I Kimura
November 1975,
Research in experimental medicine. Zeitschrift fur die gesamte experimentelle Medizin einschliesslich experimenteller Chirurgie,
P M Chen, and
N Yamanaka, and
K Kikuchi, and
K Kitajima, and
I Kimura
September 1977,
Transplantation,
P M Chen, and
N Yamanaka, and
K Kikuchi, and
K Kitajima, and
I Kimura
January 1975,
International archives of allergy and applied immunology,
P M Chen, and
N Yamanaka, and
K Kikuchi, and
K Kitajima, and
I Kimura
January 1971,
Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology,
P M Chen, and
N Yamanaka, and
K Kikuchi, and
K Kitajima, and
I Kimura
May 1977,
Clinical and experimental immunology,
P M Chen, and
N Yamanaka, and
K Kikuchi, and
K Kitajima, and
I Kimura
January 1975,
International archives of allergy and applied immunology,
