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Protein kinase C (PKC) from human platelets was resolved into two fractions by hydroxyapatite column chromatography. One of the enzymes was indistinguishable from the brain type III PKC having alpha-sequence in its kinetic and immunological properties. The other enzyme was kinetically different from any of the brain PKC subspecies so far isolated, although it resembled the brain type II PKC having beta-sequence. With H1 histone as substrate, this platelet enzyme was not very sensitive to Ca2+, and activated partly by phosphatidylserine plus diacylglycerol or by free arachidonic acid. Both platelet enzymes could phosphorylate the P47 protein in vitro, but the enzyme physiologically responsible for the P47 protein phosphorylation in the activated platelets remains to be identified.
M Tsukuda, and
Y Asaoka, and
K Sekiguchi, and
U Kikkawa, and
Y Nishizuka
June 1989,
Biochemical and biophysical research communications,
M Tsukuda, and
Y Asaoka, and
K Sekiguchi, and
U Kikkawa, and
Y Nishizuka
December 1989,
Gastroenterology,
M Tsukuda, and
Y Asaoka, and
K Sekiguchi, and
U Kikkawa, and
Y Nishizuka
January 1990,
Life sciences,
M Tsukuda, and
Y Asaoka, and
K Sekiguchi, and
U Kikkawa, and
Y Nishizuka
June 1996,
Journal of cellular physiology,
M Tsukuda, and
Y Asaoka, and
K Sekiguchi, and
U Kikkawa, and
Y Nishizuka
January 1985,
Advances in experimental medicine and biology,
M Tsukuda, and
Y Asaoka, and
K Sekiguchi, and
U Kikkawa, and
Y Nishizuka
October 1997,
Thrombosis research,
M Tsukuda, and
Y Asaoka, and
K Sekiguchi, and
U Kikkawa, and
Y Nishizuka
April 1989,
FEBS letters,
M Tsukuda, and
Y Asaoka, and
K Sekiguchi, and
U Kikkawa, and
Y Nishizuka
October 1992,
The Kobe journal of medical sciences,
M Tsukuda, and
Y Asaoka, and
K Sekiguchi, and
U Kikkawa, and
Y Nishizuka
January 1985,
Reviews in clinical & basic pharmacology,
M Tsukuda, and
Y Asaoka, and
K Sekiguchi, and
U Kikkawa, and
Y Nishizuka
December 1998,
Japanese journal of pharmacology,
