Characterisation of nerve-mediated ATP release from bladder detrusor muscle and its pathological implications. 2019

Carly J McCarthy, and Youko Ikeda, and Deborah Skennerton, and Basu Chakrabarty, and Anthony J Kanai, and Rita I Jabr, and Christopher H Fry
Instituto de Investigaciones en Medicina Traslacional (IIMT), Facultad de Ciencias Biomédicas, Austral University, Buenos Aires, Argentina.

This study aims to characterise the molecular mechanisms that determine variability of atropine resistance of nerve-mediated contractions in human and guinea pig detrusor smooth muscle. Atropine resistance of nerve-mediated contractions and the role of P2X1 receptors, were assessed in isolated preparations from guinea pigs and also humans with or without overactive bladder syndrome, from which the mucosa was removed. Nerve-mediated ATP release was measured directly with amperometric ATP-sensitive electrodes. Ecto-ATPase activity of guinea pig and human detrusor samples was measured in vitro by measuring the concentration-dependent rate of ATP breakdown. The transcription of ecto-ATPase subtypes in human samples was measured by qPCR. Atropine resistance was greatest in guinea pig detrusor, absent in human tissue from normally functioning bladders, and intermediate in human overactive bladder. Greater atropine resistance correlated with reduction of contractions by the ATP-diphosphohydrolase apyrase, directly implicating ATP in their generation. E-NTPDase-1 was the most abundantly transcribed ecto-ATPase of those tested, and transcription was reduced in tissue from human overactive, compared to normal, bladders. E-NTPDase-1 enzymic activity was inversely related to the magnitude of atropine resistance. Nerve-mediated ATP release was continually measured and varied with stimulation frequency over the range of 1-16 Hz. Atropine resistance in nerve-mediated detrusor contractions is due to ATP release and its magnitude is inversely related to E-NTPDase-1 activity. ATP is released under different stimulation conditions compared with ACh, implying different routes for their release.

UI MeSH Term Description Entries
D009119 Muscle Contraction A process leading to shortening and/or development of tension in muscle tissue. Muscle contraction occurs by a sliding filament mechanism whereby actin filaments slide inward among the myosin filaments. Inotropism,Muscular Contraction,Contraction, Muscle,Contraction, Muscular,Contractions, Muscle,Contractions, Muscular,Inotropisms,Muscle Contractions,Muscular Contractions
D009130 Muscle, Smooth Unstriated and unstriped muscle, one of the muscles of the internal organs, blood vessels, hair follicles, etc. Contractile elements are elongated, usually spindle-shaped cells with centrally located nuclei. Smooth muscle fibers are bound together into sheets or bundles by reticular fibers and frequently elastic nets are also abundant. (From Stedman, 25th ed) Muscle, Involuntary,Smooth Muscle,Involuntary Muscle,Involuntary Muscles,Muscles, Involuntary,Muscles, Smooth,Smooth Muscles
D001743 Urinary Bladder A musculomembranous sac along the URINARY TRACT. URINE flows from the KIDNEYS into the bladder via the ureters (URETER), and is held there until URINATION. Bladder,Bladder Detrusor Muscle,Detrusor Urinae,Bladder Detrusor Muscles,Bladder, Urinary,Detrusor Muscle, Bladder,Detrusor Muscles, Bladder
D004558 Electric Stimulation Use of electric potential or currents to elicit biological responses. Stimulation, Electric,Electrical Stimulation,Electric Stimulations,Electrical Stimulations,Stimulation, Electrical,Stimulations, Electric,Stimulations, Electrical
D006168 Guinea Pigs A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research. Cavia,Cavia porcellus,Guinea Pig,Pig, Guinea,Pigs, Guinea
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000251 Adenosine Triphosphatases A group of enzymes which catalyze the hydrolysis of ATP. The hydrolysis reaction is usually coupled with another function such as transporting Ca(2+) across a membrane. These enzymes may be dependent on Ca(2+), Mg(2+), anions, H+, or DNA. ATPases,Adenosinetriphosphatase,ATPase,ATPase, DNA-Dependent,Adenosine Triphosphatase,DNA-Dependent ATPase,DNA-Dependent Adenosinetriphosphatases,ATPase, DNA Dependent,Adenosinetriphosphatases, DNA-Dependent,DNA Dependent ATPase,DNA Dependent Adenosinetriphosphatases,Triphosphatase, Adenosine
D000255 Adenosine Triphosphate An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter. ATP,Adenosine Triphosphate, Calcium Salt,Adenosine Triphosphate, Chromium Salt,Adenosine Triphosphate, Magnesium Salt,Adenosine Triphosphate, Manganese Salt,Adenylpyrophosphate,CaATP,CrATP,Manganese Adenosine Triphosphate,MgATP,MnATP,ATP-MgCl2,Adenosine Triphosphate, Chromium Ammonium Salt,Adenosine Triphosphate, Magnesium Chloride,Atriphos,Chromium Adenosine Triphosphate,Cr(H2O)4 ATP,Magnesium Adenosine Triphosphate,Striadyne,ATP MgCl2
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001285 Atropine An alkaloid, originally from Atropa belladonna, but found in other plants, mainly SOLANACEAE. Hyoscyamine is the 3(S)-endo isomer of atropine. AtroPen,Atropin Augenöl,Atropine Sulfate,Atropine Sulfate Anhydrous,Atropinol,Anhydrous, Atropine Sulfate,Augenöl, Atropin,Sulfate Anhydrous, Atropine,Sulfate, Atropine

Related Publications

Carly J McCarthy, and Youko Ikeda, and Deborah Skennerton, and Basu Chakrabarty, and Anthony J Kanai, and Rita I Jabr, and Christopher H Fry
April 2022, Experimental physiology,
Carly J McCarthy, and Youko Ikeda, and Deborah Skennerton, and Basu Chakrabarty, and Anthony J Kanai, and Rita I Jabr, and Christopher H Fry
January 2011, Frontiers in pharmacology,
Carly J McCarthy, and Youko Ikeda, and Deborah Skennerton, and Basu Chakrabarty, and Anthony J Kanai, and Rita I Jabr, and Christopher H Fry
November 1993, British journal of clinical pharmacology,
Carly J McCarthy, and Youko Ikeda, and Deborah Skennerton, and Basu Chakrabarty, and Anthony J Kanai, and Rita I Jabr, and Christopher H Fry
January 1999, Progress in brain research,
Carly J McCarthy, and Youko Ikeda, and Deborah Skennerton, and Basu Chakrabarty, and Anthony J Kanai, and Rita I Jabr, and Christopher H Fry
November 2013, BJU international,
Carly J McCarthy, and Youko Ikeda, and Deborah Skennerton, and Basu Chakrabarty, and Anthony J Kanai, and Rita I Jabr, and Christopher H Fry
January 1996, The Journal of urology,
Carly J McCarthy, and Youko Ikeda, and Deborah Skennerton, and Basu Chakrabarty, and Anthony J Kanai, and Rita I Jabr, and Christopher H Fry
July 2014, The Journal of urology,
Carly J McCarthy, and Youko Ikeda, and Deborah Skennerton, and Basu Chakrabarty, and Anthony J Kanai, and Rita I Jabr, and Christopher H Fry
January 1999, Experimental physiology,
Carly J McCarthy, and Youko Ikeda, and Deborah Skennerton, and Basu Chakrabarty, and Anthony J Kanai, and Rita I Jabr, and Christopher H Fry
December 1978, Biochemistry,
Carly J McCarthy, and Youko Ikeda, and Deborah Skennerton, and Basu Chakrabarty, and Anthony J Kanai, and Rita I Jabr, and Christopher H Fry
August 1969, British journal of urology,
Copied contents to your clipboard!