Involvement of Spinal Cannabinoid CB2 Receptors in Exercise-Induced Antinociception. 2019

Rafaela Silva Dos Santos, and Carlos Arterio Sorgi, and Ana Paula Ferranti Peti, and Flávio Protasio Veras, and Lúcia Helena Faccioli, and Giovane Galdino
Institute of Motricity Sciences, Federal University of Alfenas, Minas Gerais, Av. Jovino Fernandes Sales, 2600, 37133-840, Alfenas, Brazil.

Muscle pain affects approximately 11-24% of the global population. Several studies have shown that exercise is a non-pharmacological therapy to pain control. It has been suggested that the endocannabinoid system is involved in this antinociceptive effect. However, the participation of this pathway is unclear. The present study aimed to investigate whether spinal cannabinoid CB2 receptors participate in the exercise-induced antinociception. The inflammatory muscle pain model was induced by the intramuscular injection of carrageenan. Tactile allodynia and thermal hyperalgesia were determined with the von Frey filaments and hot-plate tests. C57BL/6J female mice underwent a swimming training protocol that lasted 3 weeks. This protocol of exercise reduced carrageenan-induced tactile allodynia and thermal hyperalgesia and this effect was prevented by the cannabinoid CB2 receptors inverse agonist AM630 and potentiated by MAFP (inhibitor of the enzyme that metabolizes endocannabinoids) and minocycline (microglia inhibitor). In addition, exercise increased the endocannabinoid anandamide levels and cannabinoid CB2 receptors expression whereas it reduced Iba1 (microglial marker) protein expression as well as pro-inflammatory cytokines (TNF-α and IL-1β) in the spinal cord of mice with inflammatory muscle pain. Swimming training also reduced muscle temperature of carrageen-treated animals. The present study suggests that activation of spinal cannabinoid CB2 receptors and reduction of activated microglia are involved in exercise-induced antinociception.

UI MeSH Term Description Entries
D007211 Indoles Benzopyrroles with the nitrogen at the number one carbon adjacent to the benzyl portion, in contrast to ISOINDOLES which have the nitrogen away from the six-membered ring.
D008810 Mice, Inbred C57BL One of the first INBRED MOUSE STRAINS to be sequenced. This strain is commonly used as genetic background for transgenic mouse models. Refractory to many tumors, this strain is also preferred model for studying role of genetic variations in development of diseases. Mice, C57BL,Mouse, C57BL,Mouse, Inbred C57BL,C57BL Mice,C57BL Mice, Inbred,C57BL Mouse,C57BL Mouse, Inbred,Inbred C57BL Mice,Inbred C57BL Mouse
D010146 Pain An unpleasant sensation induced by noxious stimuli which are detected by NERVE ENDINGS of NOCICEPTIVE NEURONS. Suffering, Physical,Ache,Pain, Burning,Pain, Crushing,Pain, Migratory,Pain, Radiating,Pain, Splitting,Aches,Burning Pain,Burning Pains,Crushing Pain,Crushing Pains,Migratory Pain,Migratory Pains,Pains, Burning,Pains, Crushing,Pains, Migratory,Pains, Radiating,Pains, Splitting,Physical Suffering,Physical Sufferings,Radiating Pain,Radiating Pains,Splitting Pain,Splitting Pains,Sufferings, Physical
D010147 Pain Measurement Scales, questionnaires, tests, and other methods used to assess pain severity and duration in patients or experimental animals to aid in diagnosis, therapy, and physiological studies. Analgesia Tests,Analogue Pain Scale,Formalin Test,McGill Pain Questionnaire,Nociception Tests,Pain Assessment,Pain Intensity,Pain Severity,Tourniquet Pain Test,Visual Analogue Pain Scale,Analog Pain Scale,Assessment, Pain,McGill Pain Scale,Visual Analog Pain Scale,Analgesia Test,Analog Pain Scales,Analogue Pain Scales,Formalin Tests,Intensity, Pain,Measurement, Pain,Nociception Test,Pain Assessments,Pain Intensities,Pain Measurements,Pain Questionnaire, McGill,Pain Scale, Analog,Pain Scale, Analogue,Pain Scale, McGill,Pain Severities,Pain Test, Tourniquet,Questionnaire, McGill Pain,Scale, Analog Pain,Scale, Analogue Pain,Scale, McGill Pain,Severity, Pain,Test, Analgesia,Test, Formalin,Test, Nociception,Test, Tourniquet Pain,Tests, Nociception,Tourniquet Pain Tests
D010805 Physical Conditioning, Animal Diet modification and physical exercise to improve the ability of animals to perform physical activities. Animal Physical Conditioning,Animal Physical Conditionings,Conditioning, Animal Physical,Conditionings, Animal Physical,Physical Conditionings, Animal
D010880 Piperidines A family of hexahydropyridines.
D005260 Female Females
D006930 Hyperalgesia An increased sensation of pain or discomfort produced by minimally noxious stimuli due to damage to soft tissue containing NOCICEPTORS or injury to a peripheral nerve. Hyperalgesia, Tactile,Hyperalgesia, Thermal,Hyperalgia,Hyperalgia, Mechanical,Hyperalgia, Primary,Hyperalgia, Secondary,Allodynia,Allodynia, Mechanical,Allodynia, Tactile,Allodynia, Thermal,Hyperalgesia, Mechanical,Hyperalgesia, Primary,Hyperalgesia, Secondary,Hyperalgesic Sensations,Mechanical Allodynia,Mechanical Hyperalgesia,Tactile Allodynia,Thermal Allodynia,Allodynias,Hyperalgesias,Hyperalgesias, Thermal,Hyperalgesic Sensation,Mechanical Hyperalgia,Mechanical Hyperalgias,Primary Hyperalgia,Primary Hyperalgias,Secondary Hyperalgia,Secondary Hyperalgias,Sensation, Hyperalgesic,Sensations, Hyperalgesic,Thermal Hyperalgesia
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001095 Arachidonic Acids Eicosatetraenoic Acids,Acids, Arachidonic,Acids, Eicosatetraenoic

Related Publications

Rafaela Silva Dos Santos, and Carlos Arterio Sorgi, and Ana Paula Ferranti Peti, and Flávio Protasio Veras, and Lúcia Helena Faccioli, and Giovane Galdino
May 2006, Pain,
Rafaela Silva Dos Santos, and Carlos Arterio Sorgi, and Ana Paula Ferranti Peti, and Flávio Protasio Veras, and Lúcia Helena Faccioli, and Giovane Galdino
September 2001, Pain,
Rafaela Silva Dos Santos, and Carlos Arterio Sorgi, and Ana Paula Ferranti Peti, and Flávio Protasio Veras, and Lúcia Helena Faccioli, and Giovane Galdino
May 2013, European journal of pain (London, England),
Rafaela Silva Dos Santos, and Carlos Arterio Sorgi, and Ana Paula Ferranti Peti, and Flávio Protasio Veras, and Lúcia Helena Faccioli, and Giovane Galdino
January 1990, NIDA research monograph,
Rafaela Silva Dos Santos, and Carlos Arterio Sorgi, and Ana Paula Ferranti Peti, and Flávio Protasio Veras, and Lúcia Helena Faccioli, and Giovane Galdino
January 1995, Life sciences,
Rafaela Silva Dos Santos, and Carlos Arterio Sorgi, and Ana Paula Ferranti Peti, and Flávio Protasio Veras, and Lúcia Helena Faccioli, and Giovane Galdino
October 2013, Neuroscience,
Rafaela Silva Dos Santos, and Carlos Arterio Sorgi, and Ana Paula Ferranti Peti, and Flávio Protasio Veras, and Lúcia Helena Faccioli, and Giovane Galdino
August 1991, The Journal of pharmacology and experimental therapeutics,
Rafaela Silva Dos Santos, and Carlos Arterio Sorgi, and Ana Paula Ferranti Peti, and Flávio Protasio Veras, and Lúcia Helena Faccioli, and Giovane Galdino
April 2022, Behavioural pharmacology,
Rafaela Silva Dos Santos, and Carlos Arterio Sorgi, and Ana Paula Ferranti Peti, and Flávio Protasio Veras, and Lúcia Helena Faccioli, and Giovane Galdino
October 2001, European journal of pharmacology,
Rafaela Silva Dos Santos, and Carlos Arterio Sorgi, and Ana Paula Ferranti Peti, and Flávio Protasio Veras, and Lúcia Helena Faccioli, and Giovane Galdino
November 2007, Behavioural pharmacology,
Copied contents to your clipboard!