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The resonance Raman spectrum of the carotenoid neurosporene is shown to be a sensitive monitor of absorption shifts, and thus changes in membrane potential, in chromatophores of the GlC mutant of Rhodopseudomonas sphaeroides. For a Raman excitation wavelength at 472.7 nm, the intensities of the two most prominent resonance Raman features (v1 and v2) respond very differently to small shifts in the absorption maxima. Thus, the ratio intensity v1/intensity v2 is a sensitive probe for absorption shifts. Changes in this ratio of approximately 20% were observed during a valinomycin induced diffusion potential. At 5 degrees C changes in the average intensity ratio of +6, -4 and -14% were brought about by oligomycin, FCCP and sodium deoxycholate, respectively. The changes in intensity ratio were temperature dependent and, in addition, effects due to the laser beam acting as an actinic light could be detected. Oscillatory changes were observed in absolute Raman and Rayleigh scattering intensities for chromatophores at 5 degrees C and for intact cells under growing conditions.
Y Koyama, and
R A Long, and
W G Martin, and
P R Carey
May 1980,
Archives of biochemistry and biophysics,
Y Koyama, and
R A Long, and
W G Martin, and
P R Carey
June 1979,
FEBS letters,
Y Koyama, and
R A Long, and
W G Martin, and
P R Carey
March 1980,
The Journal of biological chemistry,
Y Koyama, and
R A Long, and
W G Martin, and
P R Carey
March 1977,
Biochimica et biophysica acta,
Y Koyama, and
R A Long, and
W G Martin, and
P R Carey
April 1979,
The Biochemical journal,
Y Koyama, and
R A Long, and
W G Martin, and
P R Carey
October 1975,
Biochimica et biophysica acta,
Y Koyama, and
R A Long, and
W G Martin, and
P R Carey
June 1977,
Biochimica et biophysica acta,
Y Koyama, and
R A Long, and
W G Martin, and
P R Carey
September 1978,
Journal of biochemistry,
Y Koyama, and
R A Long, and
W G Martin, and
P R Carey
January 1978,
Antonie van Leeuwenhoek,
Y Koyama, and
R A Long, and
W G Martin, and
P R Carey
May 1979,
FEBS letters,
