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Some properties of the receptor for IgM on human lymphocytes have been investigated. It was shown that the interaction of native IgM with the receptor present on T and B lymphocytes is not critical for its detection in the EAM-rosette assay. In fact, high values of EAM-RFC could be found on cell suspensions cultured overnight in either IgM-free or IgM-containing media. In addition, the inhibition of EAM-rosettes by human monoclonal IgM at 37 degrees C was not as effective as at 4 degrees C. Rabbit IgM showed a significantly greater ability to inhibit the binding of antigen-IgM antibody complexes than human IgM. The receptor for IgM was easily removed by handling procedures, the incubation of lymphocytes at 4 degrees C and treatment of the cells with low concentrations of trypsin or pronase. After the enzymatic treatment, a rapid resynthesis occurred, which restored the number of EAM-rosettes formed by T cells and significantly increased the number formed by B cells. The interaction between the receptor and antigen-IgM antibody complexes stopped the spontaneous shedding of the receptor at 4 degrees C. When the incubation of the cells with immune complexes was performed at 37 degrees C, a significantly different behaviour between T cells equipped with receptor for IgM and those possessing receptor for IgG was found. After the binding of EAG to the receptor for IgG, a process of rapid dissociation of rosettes occurred, whereas the incubation with EAM did not induce an irreversible loss of the receptor for IgM.
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S Romagnani, and
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January 2022,
ImmunoHorizons,
S Romagnani, and
E Maggi, and
M Lorenzini, and
G M Giudizi, and
R Biagiotti, and
M Ricci
September 1979,
Clinical and experimental immunology,
S Romagnani, and
E Maggi, and
M Lorenzini, and
G M Giudizi, and
R Biagiotti, and
M Ricci
June 1976,
Immunology,
S Romagnani, and
E Maggi, and
M Lorenzini, and
G M Giudizi, and
R Biagiotti, and
M Ricci
February 1979,
Clinical and experimental immunology,
