BIOMAT Database Logo BIOMAT Database Logo

Bordet-Gengou agar medium supplemented with albumin-containing biologics for cultivation of bordetellae. 2019

Yukihiro Hiramatsu, and Mayuko Osada-Oka, and Yasuhiko Horiguchi
Department of Molecular Bacteriology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan.

Bordetella pertussis, B. parapertussis, and B. bronchiseptica cause respiratory infections in mammals, including humans, and are generally cultivated on Bordet-Gengou (BG) agar plates in laboratories. The medium requires animal blood as a supplement for better bacterial growth. However, using blood is problematic, as its constant supply is occasionally difficult because of the limited shelf-life. This study proposes modified BG agar plates supplemented with bovine serum albumin and fetal bovine serum as a simple and convenient medium that confers sufficient growth of bordetellae.

UI MeSH Term Description Entries
D001886 Bordetella pertussis A species of gram-negative, aerobic bacteria that is the causative agent of WHOOPING COUGH. Its cells are minute coccobacilli that are surrounded by a slime sheath. Bacterium tussis-convulsivae,Haemophilus pertussis,Hemophilus pertussis
D003470 Culture Media Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN. Media, Culture
D000362 Agar A complex sulfated polymer of galactose units, extracted from Gelidium cartilagineum, Gracilaria confervoides, and related red algae. It is used as a gel in the preparation of solid culture media for microorganisms, as a bulk laxative, in making emulsions, and as a supporting medium for immunodiffusion and immunoelectrophoresis.
D001688 Biological Products Complex pharmaceutical substances, preparations, or matter derived from organisms usually obtained by biological methods or assay. Biologic,Biologic Drug,Biologic Product,Biological,Biological Drug,Biological Medicine,Biological Product,Biologics,Biopharmaceutical,Natural Product,Natural Products,Biologic Drugs,Biologic Medicines,Biologic Pharmaceuticals,Biologic Products,Biological Drugs,Biological Medicines,Biologicals,Biopharmaceuticals,Products, Biological,Drug, Biologic,Drug, Biological,Drugs, Biologic,Drugs, Biological,Medicine, Biological,Medicines, Biologic,Medicines, Biological,Pharmaceuticals, Biologic,Product, Biologic,Product, Biological,Product, Natural
D012710 Serum Albumin, Bovine Serum albumin from cows, commonly used in in vitro biological studies. (From Stedman, 25th ed) Fetal Bovine Serum,Fetal Calf Serum,Albumin Bovine,Bovine Albumin,Bovine Serum Albumin,Albumin, Bovine,Albumin, Bovine Serum,Bovine Serum, Fetal,Bovine, Albumin,Calf Serum, Fetal,Serum, Fetal Bovine,Serum, Fetal Calf
D016950 Bordetella bronchiseptica A species of BORDETELLA that is parasitic and pathogenic. It is found in the respiratory tract of domestic and wild mammalian animals and can be transmitted from animals to man. It is a common cause of bronchopneumonia in lower animals. Alcaligenes bronchicanis,Alcaligenes bronchisepticus,Bacillus bronchicanis,Bacillus bronchisepticus,Bacterium bronchisepticus,Brucella bronchiseptica,Haemophilus bronchisepticus
D042483 Bordetella parapertussis A species of BORDETELLA with similar morphology to BORDETELLA PERTUSSIS, but growth is more rapid. It is found only in the RESPIRATORY TRACT of humans.

Related Publications

Yukihiro Hiramatsu, and Mayuko Osada-Oka, and Yasuhiko Horiguchi
[Prolonged cultivation of whooping cough microbes of the phase I on the casein-charcoal agar and Bordet-Gengou medium].
November 1961, Zhurnal mikrobiologii, epidemiologii i immunobiologii,
Yukihiro Hiramatsu, and Mayuko Osada-Oka, and Yasuhiko Horiguchi
Observations on the value of the Bordet-Gengou medium for the cultivation of Haemophilus pertussis.
May 1951, Journal of general microbiology,
Yukihiro Hiramatsu, and Mayuko Osada-Oka, and Yasuhiko Horiguchi
Comparison of cyclodextrin solid medium containing blood with Bordet-Gengou medium for growth of Bordetella pertussis phase I organisms.
March 1991, Vaccine,
Yukihiro Hiramatsu, and Mayuko Osada-Oka, and Yasuhiko Horiguchi
Use of cow's blood in Bordet-Gengou medium for pertussis vaccine preparation.
March 1956, Applied microbiology,
Yukihiro Hiramatsu, and Mayuko Osada-Oka, and Yasuhiko Horiguchi
Comparison of blood-free medium (cyclodextrin solid medium) with Bordet-Gengou medium for clinical isolation of Bordetella pertussis.
June 1986, Journal of clinical microbiology,
Yukihiro Hiramatsu, and Mayuko Osada-Oka, and Yasuhiko Horiguchi
Isolation of Bordetella pertussis: benefits of using both Bordet-Gengou and charcoal agar media.
September 1984, Journal of clinical pathology,
Yukihiro Hiramatsu, and Mayuko Osada-Oka, and Yasuhiko Horiguchi
THE BORDET-GENGOU BACILLUS OF PERTUSSIS.
January 1909, The Journal of experimental medicine,
Yukihiro Hiramatsu, and Mayuko Osada-Oka, and Yasuhiko Horiguchi
Shelf life of prepared Bordet-Gengou and Regan-Lowe agar plates for isolation of Bordetella pertussis.
November 1991, European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology,
Yukihiro Hiramatsu, and Mayuko Osada-Oka, and Yasuhiko Horiguchi
SERUM ALBUMIN SUPPLEMENTED MEDIUM FOR LONG TERM CULTIVATION OF MAMMALIAN FIBROBLAST STRAINS.
July 1964, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
Yukihiro Hiramatsu, and Mayuko Osada-Oka, and Yasuhiko Horiguchi
[Biological diagnosis of whooping cough by isolation of the Bordet-Gengou bacillus (H. pertussis) on modified Bordet medium].
January 1950, Annales de biologie clinique,
Copied contents to your clipboard!