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Establishment of a cell line from egg of rare minnow Gobiocypris rarus for propagation of grass carp reovirus genotype II. 2019

Shixu Liu, and Yingying Wang, and Jiaming Chen, and Qing Wang, and Ouqin Chang, and Weiwei Zeng, and Sven M Bergmann, and Yingying Li, and Jiyuan Yin, and Hong Wen
Key Lab of Fishery Drug Development, Ministry of Agriculture, Key Lab of Aquatic Animal Immune Technology, Peal River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510380, China; College of Fisheries, Tianjin Agricultural University, Tianjin, 300384, China.

The rare minnow, Gobiocypris rarus, is small experimental fish proven to be sensitive to Grass Carp Reovirus (GCRV) infection. In present study we established a new cell (GrE) from eggs of G. rarus. GrE cells grew well at 28 °C in M199 medium containing 10% fetal bovine serum, and has been subcultured for over 70 passages. Chromosome analysis indicated that 40% of the cells were diploid 2n = 66 while the chromosome number of the fish is 2n = 50. Viral replication in GrE cells was confirmed by transmission electron microscopy, immunofluorescence assays and virus titration experiments. GrE cells and Cyenopharyngodon idellus kidney cells were infected with two GCRV genotypes while the virus copies of GCRV II in GrE peaked at 2.25 × 105 on 12th dpi. In vivo challenge experiments using GCRV I and II isolates at generations 1 and 20 indicated that GCRV II reproduce similar symptoms and histopathological changes of the disease in the rare minnow. These results indicated that GrE is permissive for GCRV genotype II propagation and can be used for pathogenesis studies and vaccine development of the predominant genotype of GCRV.

UI MeSH Term Description Entries
D012088 Reoviridae Infections Infections produced by reoviruses, general or unspecified. Reovirus Infections,Infections, Reoviridae,Infection, Reoviridae,Infection, Reovirus,Infections, Reovirus,Reoviridae Infection,Reovirus Infection
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D003470 Culture Media Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN. Media, Culture
D003530 Cyprinidae A family of freshwater fish comprising the minnows or CARPS. Barbels,Chub,Dace,Minnows,Roach (Fish),Shiner,Tench,Tinca,Barbus,Rutilus rutilus,Tinca tinca,Chubs,Shiners,Tinca tincas,tinca, Tinca
D004171 Diploidy The chromosomal constitution of cells, in which each type of CHROMOSOME is represented twice. Symbol: 2N or 2X. Diploid,Diploid Cell,Cell, Diploid,Cells, Diploid,Diploid Cells,Diploidies,Diploids
D005393 Fish Diseases Diseases of freshwater, marine, hatchery or aquarium fish. This term includes diseases of both teleosts (true fish) and elasmobranchs (sharks, rays and skates). Disease, Fish,Diseases, Fish,Fish Disease
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D014776 Virus Cultivation Process of growing viruses in live animals, plants, or cultured cells. Viral Cultivation,Cultivation, Viral,Cultivation, Virus,Cultivations, Viral,Cultivations, Virus,Viral Cultivations,Virus Cultivations
D030102 Orthoreovirus, Mammalian A species of ORTHOREOVIRUS infecting mammals (other than baboons). There are four serotypes. In humans they are generally benign but may sometimes cause upper respiratory tract illness or enteritis in infants and children. MAMMALIAN ORTHOREOVIRUS 3 is a very pathogenic virus in laboratory rodents. Reovirus 1,Reovirus 2,Mammalian Orthoreovirus,Mammalian Orthoreovirus 1,Mammalian Orthoreovirus 2,Mammalian Reovirus 1,Mammalian Reovirus 2,Reovirus Type 1,Reovirus Type 2,Mammalian Orthoreoviruses,Mammalian Reovirus 1s,Orthoreoviruses, Mammalian

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