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This study reports the adaptation of an electronic particle-counting method for the enumeration of T cell and B cell rosettes of human lymphocytes. This method and the conventional hemocytometer method were compared in parallel on seventeen T cell and seven B cell rosette enumerations in a blind study design. All data were analyzed for statistical significance; correlation coefficents were calculated. There was significant agreement between both methods for T cells at p less than 0.001 (mean particle counter 80.6% +/-11.3 mean hemocytometer 76.7+/-8.2%) and for the B cells at p less than 0.002 (mean particle counter 22.5+/-9.7, mean hemocytometer 23.4+/-5.12). These results demonstrate that the electronic particle counter is useful as a rapid, convenient, semi-automated method for the enumeration of T cell and B cell rosettes.
T N Denny, and
K M Kinsley, and
J W Benson, and
R W Bull
March 1976,
Journal of immunological methods,
T N Denny, and
K M Kinsley, and
J W Benson, and
R W Bull
September 1973,
The New England journal of medicine,
T N Denny, and
K M Kinsley, and
J W Benson, and
R W Bull
July 1975,
Journal of immunological methods,
T N Denny, and
K M Kinsley, and
J W Benson, and
R W Bull
January 1978,
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T N Denny, and
K M Kinsley, and
J W Benson, and
R W Bull
January 1965,
Fertility and sterility,
T N Denny, and
K M Kinsley, and
J W Benson, and
R W Bull
January 1979,
Medical laboratory sciences,
T N Denny, and
K M Kinsley, and
J W Benson, and
R W Bull
April 1958,
Blood,
T N Denny, and
K M Kinsley, and
J W Benson, and
R W Bull
February 1967,
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T N Denny, and
K M Kinsley, and
J W Benson, and
R W Bull
October 1975,
Alergia,
T N Denny, and
K M Kinsley, and
J W Benson, and
R W Bull
March 1968,
Technical bulletin of the Registry of Medical Technologists. American Society of Clinical Pathologists. Registry of Medical Technologists,
