Molecular detection of Rickettsia genus in chigger mites (Trombidiformes: Trombiculidae) collected on small mammals in southeastern brazilian. 2019

Fernando de Castro Jacinavicius, and Ricardo Bassini-Silva, and Sebastián Muñoz-Leal, and Cal Welbourn, and Ronald Ochoa, and Marcelo Bahia Labruna, and Darci Moraes Barros-Battesti
Laboratório Especial de Coleções Zoológicas, Instituto Butantan, São Paulo, SP, Brasil.

Chiggers are ectoparasites of vertebrates and may cause trombiculiasis or transmit pathogens to their hosts. Specimens collected from rodents and marsupials were morphologically identified as Herpetacarus hertigi, Eutrombicula tinami, Kymocta sp., Quadraseta brasiliensis, Quadraseta falconensis, Quadraseta flochi, Quadraseta mackenziei, Quadraseta pazca, Quadraseta trapezoides, Quadraseta sp., Serratacarus sp., and Trombewingia bakeri. These mites were submitted individually to molecular analyses for the detection of bacteria of the genus Coxiella, Hepatozoon and Rickettsia. Samples were positive to Rickettsia only. Obtained sequences for the gltA (350 pb) and ompA (488 pb) genes were identical to "Candidatus Rickettsia colombianensi", a species previously detected in ticks. In addition, molecular identification of mites based on 18S rDNA sequences are provided for H. hertigi, Kymocta sp., Q. brasiliensis, Q. pazca, Q. trapezoides, Quadraseta sp., and T. bakeri for the first time. This is the first report of the detection of a Rickettsia sp. in chigger mites collected on rodents in Brazil.

UI MeSH Term Description Entries
D008394 Marsupialia An infraclass of MAMMALS, also called Metatheria, where the young are born at an early stage of development and continue to develop in a pouch (marsupium). In contrast to Eutheria (placentals), marsupials have an incomplete PLACENTA. Metatheria,Marsupials,Marsupial
D008924 Mite Infestations Infestations with arthropods of the subclass ACARI, superorder Acariformes. Acariasis,Mange,Infestation, Mite,Infestations, Mite,Mite Infestation
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012281 Rickettsia A genus of gram-negative, aerobic, rod-shaped bacteria often surrounded by a protein microcapsular layer and slime layer. The natural cycle of its organisms generally involves a vertebrate and an invertebrate host. Species of the genus are the etiological agents of human diseases, such as typhus.
D012329 RNA, Bacterial Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis. Bacterial RNA
D012337 RNA, Ribosomal, 18S Constituent of the 40S subunit of eukaryotic ribosomes. 18S rRNA is involved in the initiation of polypeptide synthesis in eukaryotes. 18S Ribosomal RNA,18S RRNA,RNA, 18S Ribosomal,Ribosomal RNA, 18S
D012377 Rodentia A mammalian order which consists of 29 families and many genera. Beavers,Capybaras,Castor Beaver,Dipodidae,Hydrochaeris,Jerboas,Rodents,Beaver,Capybara,Hydrochaeri,Jerboa,Rodent,Rodentias
D014324 Trombiculidae Family of MITES in the superfamily Trombiculoidea, suborder Prostigmata, which attack humans and other vertebrates, causing DERMATITIS and severe allergic reactions. Chiggers, red bugs, and harvest mites commonly refer to the larval stage of Trombiculid mites, the only parasitic stage of the mite's life cycle. Chiggers,Harvest Mites,Leptotrombidium,Neotrombicula,Red Bugs,Trombicula,Trombiculid Mites,Eutrombicula,Bug, Red,Bugs, Red,Chigger,Eutrombiculas,Harvest Mite,Leptotrombidiums,Mite, Harvest,Mite, Trombiculid,Mites, Harvest,Mites, Trombiculid,Neotrombiculas,Red Bug,Trombiculas,Trombiculid Mite
D016133 Polymerase Chain Reaction In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships. Anchored PCR,Inverse PCR,Nested PCR,PCR,Anchored Polymerase Chain Reaction,Inverse Polymerase Chain Reaction,Nested Polymerase Chain Reaction,PCR, Anchored,PCR, Inverse,PCR, Nested,Polymerase Chain Reactions,Reaction, Polymerase Chain,Reactions, Polymerase Chain

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