The frequency of occurrence and the genetic structure of polylysogens were studied for phages lambda, phi 80 and lambda att80. In the case of lambda, frequency of polylysogenization is high (0.20 to 0.41) with a tandem integration of prophages at the primary att site (att lambda). With phi 80 and lambda att80, this frequency is about 10 times lower, and usually one prophage becomes integrated at the primary att site (att80-I) while another (sometimes two others) integrates at one of the secondary sites. At least four secondary att80 sites have been found in wild-type Escherichia coli, two of which (near the his and tolC loci) are preferred. The frequency of secondary integration of phi 80 and lambda att80 does not differ significantly in the wild-type host and in that deleted for the primary att site (0.041 and 0.045, respectively, among surviving cells at an MOI of 10). Homoimmune superinfection has revealed a constitutive cI-independent expression of the phi 80 int gene in the prophage state. The only phi 80 tandem detected proved to be unstable. With the phi 80int- mutant, we observed stabilization of phi 80 tandems; as a consequence, their frequency of occurrence during coinfection with phi 80int+ was up to the lambda level and no nontandem insertions were found. A model is proposed for the phi 80 and lambda att80 nontandem integration.