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Light-sheet microscopy of cleared tissues with isotropic, subcellular resolution. 2019
Tonmoy Chakraborty, and
Meghan K Driscoll, and
Elise Jeffery, and
Malea M Murphy, and
Philippe Roudot, and
Bo-Jui Chang, and
Saumya Vora, and
Wen Mai Wong, and
Cara D Nielson, and
Hua Zhang, and
Vladimir Zhemkov, and
Chitkale Hiremath, and
Estanislao Daniel De La Cruz, and
Yating Yi, and
Ilya Bezprozvanny, and
Hu Zhao, and
Raju Tomer, and
Rainer Heintzmann, and
Julian P Meeks, and
Denise K Marciano, and
Sean J Morrison, and
Gaudenz Danuser, and
Kevin M Dean, and
Reto Fiolka
Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA.
We present cleared-tissue axially swept light-sheet microscopy (ctASLM), which enables isotropic, subcellular resolution imaging with high optical sectioning capability and a large field of view over a broad range of immersion media. ctASLM can image live, expanded, and both aqueous and non-aqueous chemically cleared tissue preparations. Depending on the optical configuration, ctASLM provides up to 260 nm of axial resolution, a three to tenfold improvement over confocal and other reported cleared-tissue light-sheet microscopes. We imaged millimeter-scale cleared tissues with subcellular three-dimensional resolution, which enabled automated detection of multicellular tissue architectures, individual cells, synaptic spines and rare cell-cell interactions.
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Saumya Vora, and
Wen Mai Wong, and
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Chitkale Hiremath, and
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Estanislao Daniel De La Cruz, and
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Ilya Bezprozvanny, and
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