Biomaterial Database

Cholesteryl ester accumulation in smooth muscle cells after uptake of necrotic products from atherosclerotic lesions. 1988

S Hashimoto, and H Wong, and D Blissard, and D Anderson

Research Service, Wadsworth Medical Center, Los Angeles, California 90073.

Cholesterol-ladened plasma membrane vesicles were used to load smooth muscle cells (SMC) with cholesterol. Plasma membrane vesicles (PMV) were isolated from rabbit atherosclerotic lesions, and characterized as to size, cholesterol content, and marker enzyme (plasma membrane, lysosome, endoplasmic reticulum) composition. PMV were regarded as a necrotic product since they are produced upon injury to cells. Degradation of PMV was proportional to the PMV protein concentration in the culture medium, suggesting bulk intake of PMV. Cholesterol accumulation of SMC varied with the cholesterol content of the vesicle. Incubation for 3 days with PMV having 0.39 and 0.62 mg cholesterol/mg protein induced the accumulation of 8 and 29 micrograms of esterified cholesterol/mg cell protein, respectively. Incorporation of oleate into cholesteryl ester during a 24-hr period under these conditions, however, was the same. The contribution of cholesterol ester synthesis to the esterified cholesterol content of SMC was 40 and 11% of the total when exposed to PMV having, respectively, low and high contents of cholesterol. This study suggests that cholesterol-bearing PMV in lesions can be utilized to load lesion-SMC. These observations suggest that lipid-bearing elements other than low density lipoprotein may be responsible for cholesterol-loaded SMC in lesions.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D009131	Muscle, Smooth, Vascular	The nonstriated involuntary muscle tissue of blood vessels.	Vascular Smooth Muscle,Muscle, Vascular Smooth,Muscles, Vascular Smooth,Smooth Muscle, Vascular,Smooth Muscles, Vascular,Vascular Smooth Muscles
D009336	Necrosis	The death of cells in an organ or tissue due to disease, injury or failure of the blood supply.
D011817	Rabbits	A burrowing plant-eating mammal with hind limbs that are longer than its fore limbs. It belongs to the family Leporidae of the order Lagomorpha, and in contrast to hares, possesses 22 instead of 24 pairs of chromosomes.	Belgian Hare,New Zealand Rabbit,New Zealand Rabbits,New Zealand White Rabbit,Rabbit,Rabbit, Domestic,Chinchilla Rabbits,NZW Rabbits,New Zealand White Rabbits,Oryctolagus cuniculus,Chinchilla Rabbit,Domestic Rabbit,Domestic Rabbits,Hare, Belgian,NZW Rabbit,Rabbit, Chinchilla,Rabbit, NZW,Rabbit, New Zealand,Rabbits, Chinchilla,Rabbits, Domestic,Rabbits, NZW,Rabbits, New Zealand,Zealand Rabbit, New,Zealand Rabbits, New,cuniculus, Oryctolagus
D002462	Cell Membrane	The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.	Plasma Membrane,Cytoplasmic Membrane,Cell Membranes,Cytoplasmic Membranes,Membrane, Cell,Membrane, Cytoplasmic,Membrane, Plasma,Membranes, Cell,Membranes, Cytoplasmic,Membranes, Plasma,Plasma Membranes
D002499	Centrifugation, Density Gradient	Separation of particles according to density by employing a gradient of varying densities. At equilibrium each particle settles in the gradient at a point equal to its density. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)	Centrifugations, Density Gradient,Density Gradient Centrifugation,Density Gradient Centrifugations,Gradient Centrifugation, Density,Gradient Centrifugations, Density
D002788	Cholesterol Esters	Fatty acid esters of cholesterol which constitute about two-thirds of the cholesterol in the plasma. The accumulation of cholesterol esters in the arterial intima is a characteristic feature of atherosclerosis.	Cholesterol Ester,Cholesteryl Ester,Cholesteryl Esters,Ester, Cholesterol,Ester, Cholesteryl,Esters, Cholesterol,Esters, Cholesteryl
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001013	Aorta, Thoracic	The portion of the descending aorta proceeding from the arch of the aorta and extending to the DIAPHRAGM, eventually connecting to the ABDOMINAL AORTA.	Aorta, Ascending,Aorta, Descending,Aortic Arch,Aortic Root,Arch of the Aorta,Descending Aorta,Sinotubular Junction,Ascending Aorta,Thoracic Aorta,Aortic Roots,Arch, Aortic,Ascending Aortas,Junction, Sinotubular,Root, Aortic,Sinotubular Junctions