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(E)-5-(2-Bromovinyl)-2'-deoxyuridine is an antiviral drug that is experimentally used for modulation of the antitumour effect of fluoropyrimidines, such as ftorafur and 5-fluorouracil. The isolation of the analyte, in the presence of 5-fluorouracil, from the matrix is performed either by means of a simple protein precipitation (plasma) or by means of a liquid-liquid extraction with ethyl acetate (urine). Following pretreatment, the analyte is analysed by reversed-phase chromatography and quantified by absorbance detection at 307 nm. The minimum detectable concentration in plasma and urine samples is ca. 6 ng/ml. The recovery after deproteination of plasma samples is 75%, while after liquid-liquid extraction of urine the recovery amounts 92%. The degree of protein binding of the analyte, measured by ultrafiltration, is found to be 97%. These data allow the bioanalysis of (E)-5-(2-bromovinyl)-2'-deoxyuridine for pharmacokinetic studies.
H J Reeuwijk, and
H Lingeman, and
U R Tjaden, and
E A de Bruijn, and
H J Keizer, and
J van der Greef
January 2005,
Medicinal research reviews,
H J Reeuwijk, and
H Lingeman, and
U R Tjaden, and
E A de Bruijn, and
H J Keizer, and
J van der Greef
May 1992,
Fundamental and applied toxicology : official journal of the Society of Toxicology,
H J Reeuwijk, and
H Lingeman, and
U R Tjaden, and
E A de Bruijn, and
H J Keizer, and
J van der Greef
August 1984,
The Journal of antimicrobial chemotherapy,
H J Reeuwijk, and
H Lingeman, and
U R Tjaden, and
E A de Bruijn, and
H J Keizer, and
J van der Greef
January 1987,
Nucleic acids symposium series,
H J Reeuwijk, and
H Lingeman, and
U R Tjaden, and
E A de Bruijn, and
H J Keizer, and
J van der Greef
August 1979,
Antimicrobial agents and chemotherapy,
H J Reeuwijk, and
H Lingeman, and
U R Tjaden, and
E A de Bruijn, and
H J Keizer, and
J van der Greef
November 1983,
Nucleic acids research,
H J Reeuwijk, and
H Lingeman, and
U R Tjaden, and
E A de Bruijn, and
H J Keizer, and
J van der Greef
November 1980,
British medical journal,
H J Reeuwijk, and
H Lingeman, and
U R Tjaden, and
E A de Bruijn, and
H J Keizer, and
J van der Greef
January 1983,
Nucleic acids symposium series,
H J Reeuwijk, and
H Lingeman, and
U R Tjaden, and
E A de Bruijn, and
H J Keizer, and
J van der Greef
December 1981,
Nucleic acids research,
H J Reeuwijk, and
H Lingeman, and
U R Tjaden, and
E A de Bruijn, and
H J Keizer, and
J van der Greef
January 1982,
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie,
