Plants subjected to drought and saline stress conditions suffer from tissue dehydration. Such dehydration is caused by the imbalance between root water uptake by roots and water loss by transpiration. Therefore, determination of root hydraulic properties is crucial to understand plant water balance. Root hydraulic conductivity (L) can be used to estimate root water transport capacity. L depends on root architecture (length and diameter of the root and proliferation of secondary roots), radial water transport pathway (root xylem vessels, plasmodesmata, apoplastic space, caspary bands), and on intrinsic membrane permeability to water (aquaporins, water membrane protein channels). Different methods have been developed to measure L, such as Pressure Chamber, Free Exudation, High-Pressure Flowmeter (HPFM), and Root Pressure Probe (RPP). In this chapter, we will focus on Pressure Chamber, Free Exudation, and HPFM methods which have been used to determine the effect of jasmonates (JA) on root hydraulic conductivity.