[Non-pigmented Serratia: utility of hydrolysis of Tween 80 in its identification]. 1988

O Riesco, and A Roncoroni, and J Smayevsky, and H Bianchini
Departamento de Patología Clínica, Clínicas Dr. Norberto Quirno, Buenos Aires, Argentina.

The ability of Serratia sp. to hydrolyze Tween 80 and the usefulness of this test for differentiating it from other Gram negative bacilli was studied, comparing it with the capacity of production of DNAsa by the same strains using the O-Toluidine blue method. The total number of strains assayed was 88. The sensitivity obtained was 83.9% and specificity 91.3%, with a positive predictive value of 83.9% and a negative predictive value of 91.2%. Using this method to differentiate only non-pigmented Serratia from Klebsiella sp. and Enterobacter sp. the sensitivity and specificity obtained was 100%. Concerning the cost, this method proved to be 3.86 times cheaper than the DNAsa one.

UI MeSH Term Description Entries
D011136 Polysorbates Sorbitan mono-9-octadecanoate poly(oxy-1,2-ethanediyl) derivatives; complex mixtures of polyoxyethylene ethers used as emulsifiers or dispersing agents in pharmaceuticals. Polysorbate,Polysorbate 20,Polysorbate 80,Sorbitan Derivatives,Tween,Tweens,PSML,Polyoxyethylene Sorbitan Monolaurate,Tween 20,Tween 60,Tween 80,Tween 81,Tween 85,20s, Polysorbate,20s, Tween,60s, Tween,80s, Polysorbate,80s, Tween,81s, Tween,85s, Tween,Derivative, Sorbitan,Derivatives, Sorbitan,Monolaurate, Polyoxyethylene Sorbitan,Monolaurates, Polyoxyethylene Sorbitan,PSMLs,Polyoxyethylene Sorbitan Monolaurates,Polysorbate 20s,Polysorbate 80s,Sorbitan Derivative,Sorbitan Monolaurate, Polyoxyethylene,Sorbitan Monolaurates, Polyoxyethylene,Tween 20s,Tween 60s,Tween 80s,Tween 81s,Tween 85s
D004269 DNA, Bacterial Deoxyribonucleic acid that makes up the genetic material of bacteria. Bacterial DNA
D006868 Hydrolysis The process of cleaving a chemical compound by the addition of a molecule of water.
D012705 Serratia A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria that occurs in the natural environment (soil, water, and plant surfaces) or as an opportunistic human pathogen.

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