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The ability of platelet activating factor (PAF) to stimulate dopamine release and modify calcium homeostasis in PC-12 cell line was studied. PAF-induced dopamine release is related to its molecular form, with only the R-form steric configuration [(R)PAF], but not its S-form or its 2-lyso derivative, effective at being active. In addition, PAF acts at very low concentrations in a dose-dependent manner (0.1-30 nM). Preincubation with PAF receptor antagonists (CV-3988 and BN52021) as well as the specific desensitization of PC-12 cells to (R)PAF abolish the (R)PAF-induced dopamine release. Several lines of evidence suggest that dopamine release is dependent on a (R)PAF-induced calcium influx and efflux modulation. Dopamine release by PC-12 cells challenged with (R)PAF is associated with a rapid 45Ca influx and efflux and a rise in cytoplasmic calcium concentrations ([Ca2+]i) evaluated by using the calcium indicators fura-2 and quin2. At 30 nM (R)PAF, the absence of extracellular calcium inhibits the dopamine release but not the rise of [Ca2+]i from the internal stores, suggesting the importance of calcium influx in (R)PAF-induced dopamine release. PAF, which has been reported to be synthesized by stimulated neuronal cells (J. Biol. Chem. 261: 16502-16508, 1986) may thus have a physiological modulatory role on cells with neurosecretory properties.
F Bussolino, and
F Tessari, and
F Turrini, and
P Braquet, and
G Camussi, and
M Prosdocimi, and
A Bosia
January 2011,
Mediators of inflammation,
F Bussolino, and
F Tessari, and
F Turrini, and
P Braquet, and
G Camussi, and
M Prosdocimi, and
A Bosia
January 2000,
The European respiratory journal,
F Bussolino, and
F Tessari, and
F Turrini, and
P Braquet, and
G Camussi, and
M Prosdocimi, and
A Bosia
December 1995,
The American journal of physiology,
F Bussolino, and
F Tessari, and
F Turrini, and
P Braquet, and
G Camussi, and
M Prosdocimi, and
A Bosia
July 1988,
Immunology,
F Bussolino, and
F Tessari, and
F Turrini, and
P Braquet, and
G Camussi, and
M Prosdocimi, and
A Bosia
May 2006,
Cancer research,
F Bussolino, and
F Tessari, and
F Turrini, and
P Braquet, and
G Camussi, and
M Prosdocimi, and
A Bosia
October 1993,
FEBS letters,
F Bussolino, and
F Tessari, and
F Turrini, and
P Braquet, and
G Camussi, and
M Prosdocimi, and
A Bosia
June 1998,
The American journal of physiology,
F Bussolino, and
F Tessari, and
F Turrini, and
P Braquet, and
G Camussi, and
M Prosdocimi, and
A Bosia
July 2002,
Journal of neuroscience research,
F Bussolino, and
F Tessari, and
F Turrini, and
P Braquet, and
G Camussi, and
M Prosdocimi, and
A Bosia
July 1995,
Molecular pharmacology,
F Bussolino, and
F Tessari, and
F Turrini, and
P Braquet, and
G Camussi, and
M Prosdocimi, and
A Bosia
December 2013,
Molecular and cellular biochemistry,
