BIOMAT Database Logo BIOMAT Database Logo

Isolation and partial sequence of recombinant plasmids containing human alpha-, beta- and gamma-globin cDNA fragments. 1978

P Little, and P Curtis, and C Coutelle, and J Van den Berg, and R Dalgleish, and S Malcolm, and M Courtney, and D Westaway, and R Williamson

Human globin cDNA-derived recombinants with plasmid pCR1 have been prepared for use as specific hybridisation probes and for the partial sequencing of alpha-, beta- and gamma-globin genes.

UI MeSH Term Description Entries
D008040 Genetic Linkage The co-inheritance of two or more non-allelic GENES due to their being located more or less closely on the same CHROMOSOME. Genetic Linkage Analysis,Linkage, Genetic,Analyses, Genetic Linkage,Analysis, Genetic Linkage,Genetic Linkage Analyses,Linkage Analyses, Genetic,Linkage Analysis, Genetic
D009693 Nucleic Acid Hybridization Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503) Genomic Hybridization,Acid Hybridization, Nucleic,Acid Hybridizations, Nucleic,Genomic Hybridizations,Hybridization, Genomic,Hybridization, Nucleic Acid,Hybridizations, Genomic,Hybridizations, Nucleic Acid,Nucleic Acid Hybridizations
D010957 Plasmids Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS. Episomes,Episome,Plasmid
D004262 DNA Restriction Enzymes Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1. Restriction Endonucleases,DNA Restriction Enzyme,Restriction Endonuclease,Endonuclease, Restriction,Endonucleases, Restriction,Enzymes, DNA Restriction,Restriction Enzyme, DNA,Restriction Enzymes, DNA
D004274 DNA, Recombinant Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected. Genes, Spliced,Recombinant DNA,Spliced Gene,Recombinant DNA Research,Recombination Joint,DNA Research, Recombinant,Gene, Spliced,Joint, Recombination,Research, Recombinant DNA,Spliced Genes
D005914 Globins A superfamily of proteins containing the globin fold which is composed of 6-8 alpha helices arranged in a characterstic HEME enclosing structure. Globin
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D012333 RNA, Messenger RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm. Messenger RNA,Messenger RNA, Polyadenylated,Poly(A) Tail,Poly(A)+ RNA,Poly(A)+ mRNA,RNA, Messenger, Polyadenylated,RNA, Polyadenylated,mRNA,mRNA, Non-Polyadenylated,mRNA, Polyadenylated,Non-Polyadenylated mRNA,Poly(A) RNA,Polyadenylated mRNA,Non Polyadenylated mRNA,Polyadenylated Messenger RNA,Polyadenylated RNA,RNA, Polyadenylated Messenger,mRNA, Non Polyadenylated

Related Publications

P Little, and P Curtis, and C Coutelle, and J Van den Berg, and R Dalgleish, and S Malcolm, and M Courtney, and D Westaway, and R Williamson
Partial sequence analysis of Xenopus alpha- and beta-globin mRNA as determined from recombinant DNA plasmids.
July 1980, Developmental biology,
P Little, and P Curtis, and C Coutelle, and J Van den Berg, and R Dalgleish, and S Malcolm, and M Courtney, and D Westaway, and R Williamson
[Construction of plasmids containing different human globin gene fragments].
December 1988, Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae,
P Little, and P Curtis, and C Coutelle, and J Van den Berg, and R Dalgleish, and S Malcolm, and M Courtney, and D Westaway, and R Williamson
Construction of two plasmids, each containing three different human globin gene fragments.
January 1987, Hemoglobin,
P Little, and P Curtis, and C Coutelle, and J Van den Berg, and R Dalgleish, and S Malcolm, and M Courtney, and D Westaway, and R Williamson
Structure of recombinant plasmids containing synthetic human foetal globin gene sequences.
September 1978, Molecular & general genetics : MGG,
P Little, and P Curtis, and C Coutelle, and J Van den Berg, and R Dalgleish, and S Malcolm, and M Courtney, and D Westaway, and R Williamson
Construction of three plasmids, each containing two or three different human globin gene fragments.
January 1988, Hemoglobin,
P Little, and P Curtis, and C Coutelle, and J Van den Berg, and R Dalgleish, and S Malcolm, and M Courtney, and D Westaway, and R Williamson
Human beta-casein: partial cDNA sequence and apparent polymorphism.
April 1989, Nucleic acids research,
P Little, and P Curtis, and C Coutelle, and J Van den Berg, and R Dalgleish, and S Malcolm, and M Courtney, and D Westaway, and R Williamson
Partial nucleotide sequence of human alpha globin mRNA derived from analysis of 32P-labeled cRNA and 125I-labeled mRNA; correlation with a globin cDNA sequence.
April 1980, The Journal of biological chemistry,
P Little, and P Curtis, and C Coutelle, and J Van den Berg, and R Dalgleish, and S Malcolm, and M Courtney, and D Westaway, and R Williamson
Isolation and characterization of recombinant clones containing the chicken adult beta-globin gene.
September 1979, The Journal of biological chemistry,
P Little, and P Curtis, and C Coutelle, and J Van den Berg, and R Dalgleish, and S Malcolm, and M Courtney, and D Westaway, and R Williamson
Brain tubulin and actin cDNA sequences: isolation of recombinant plasmids.
August 1980, Nucleic acids research,
P Little, and P Curtis, and C Coutelle, and J Van den Berg, and R Dalgleish, and S Malcolm, and M Courtney, and D Westaway, and R Williamson
Construction and identification of recombinant plasmids carrying cDNAs coding for ovine alpha S1-, alpha S2-, beta-, kappa-casein and beta-lactoglobulin. Nucleotide sequence of alpha S1-casein cDNA.
September 1985, Biochimie,
Copied contents to your clipboard!