Relationship between Helicobacter pylori Infection and Plasmacytoid and Myeloid Dendritic Cells in Peripheral Blood and Gastric Mucosa of Children. 2019

Anna Helmin-Basa, and Małgorzata Wiese-Szadkowska, and Anna Szaflarska-Popławska, and Maciej Kłosowski, and Milena Januszewska, and Magdalena Bodnar, and Andrzej Marszałek, and Lidia Gackowska, and Jacek Michalkiewicz
Department of Immunology, Collegium Medicum Nicolaus Copernicus University, Bydgoszcz 85-094, Poland.

OBJECTIVE To investigate the frequency and activation status of peripheral plasmacytoid DCs (pDCs) and myeloid DCs (mDCs) as well as gastric mucosa DC subset distribution in Helicobacter pylori- (H. pylori-) infected and noninfected children. METHODS Thirty-six children were studied; twenty-one had H. pylori. The frequencies of circulating pDCs (lineage-HLA-DR+CD123+) and mDCs (lineage-HLA-DR+CD11c+) and their activation status (CD83, CD86, and HLA-DR expression) were assessed by flow cytometry. Additionally, the densities of CD11c+, CD123+, CD83+, CD86+, and LAMP3+ cells in the gastric mucosa were determined by immunohistochemistry. RESULTS The frequency of circulating CD83+ mDCs was higher in H. pylori-infected children than in the noninfected controls. The pDCs demonstrated upregulated HLA-DR surface expression, but no change in CD86 expression. Additionally, the densities of gastric lamina propria CD11c+ cells and epithelial pDCs were increased. There was a significant association between frequency of circulating CD83+ mDCs and gastric lamina propria mDC infiltration. CONCLUSIONS This study shows that although H. pylori-infected children had an increased population of mature mDCs bearing CD83 in the peripheral blood, they lack mature CD83+ mDCs in the gastric mucosa, which may promote tolerance to local antigens rather than immunity. In addition, this may reduce excessive inflammatory activity as reported for children compared to adults.

UI MeSH Term Description Entries
D007136 Immunoglobulins Multi-subunit proteins which function in IMMUNITY. They are produced by B LYMPHOCYTES from the IMMUNOGLOBULIN GENES. They are comprised of two heavy (IMMUNOGLOBULIN HEAVY CHAINS) and two light chains (IMMUNOGLOBULIN LIGHT CHAINS) with additional ancillary polypeptide chains depending on their isoforms. The variety of isoforms include monomeric or polymeric forms, and transmembrane forms (B-CELL ANTIGEN RECEPTORS) or secreted forms (ANTIBODIES). They are divided by the amino acid sequence of their heavy chains into five classes (IMMUNOGLOBULIN A; IMMUNOGLOBULIN D; IMMUNOGLOBULIN E; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M) and various subclasses. Globulins, Immune,Immune Globulin,Immune Globulins,Immunoglobulin,Globulin, Immune
D007150 Immunohistochemistry Histochemical localization of immunoreactive substances using labeled antibodies as reagents. Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D008562 Membrane Glycoproteins Glycoproteins found on the membrane or surface of cells. Cell Surface Glycoproteins,Surface Glycoproteins,Cell Surface Glycoprotein,Membrane Glycoprotein,Surface Glycoprotein,Glycoprotein, Cell Surface,Glycoprotein, Membrane,Glycoprotein, Surface,Glycoproteins, Cell Surface,Glycoproteins, Membrane,Glycoproteins, Surface,Surface Glycoprotein, Cell,Surface Glycoproteins, Cell
D003713 Dendritic Cells Specialized cells of the hematopoietic system that have branch-like extensions. They are found throughout the lymphatic system, and in non-lymphoid tissues such as SKIN and the epithelia of the intestinal, respiratory, and reproductive tracts. They trap and process ANTIGENS, and present them to T-CELLS, thereby stimulating CELL-MEDIATED IMMUNITY. They are different from the non-hematopoietic FOLLICULAR DENDRITIC CELLS, which have a similar morphology and immune system function, but with respect to humoral immunity (ANTIBODY PRODUCTION). Dendritic Cells, Interdigitating,Interdigitating Cells,Plasmacytoid Dendritic Cells,Veiled Cells,Dendritic Cells, Interstitial,Dendritic Cells, Plasmacytoid,Interdigitating Dendritic Cells,Interstitial Dendritic Cells,Cell, Dendritic,Cell, Interdigitating,Cell, Interdigitating Dendritic,Cell, Interstitial Dendritic,Cell, Plasmacytoid Dendritic,Cell, Veiled,Cells, Dendritic,Cells, Interdigitating,Cells, Interdigitating Dendritic,Cells, Interstitial Dendritic,Cells, Plasmacytoid Dendritic,Cells, Veiled,Dendritic Cell,Dendritic Cell, Interdigitating,Dendritic Cell, Interstitial,Dendritic Cell, Plasmacytoid,Interdigitating Cell,Interdigitating Dendritic Cell,Interstitial Dendritic Cell,Plasmacytoid Dendritic Cell,Veiled Cell
D005434 Flow Cytometry Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake. Cytofluorometry, Flow,Cytometry, Flow,Flow Microfluorimetry,Fluorescence-Activated Cell Sorting,Microfluorometry, Flow,Cell Sorting, Fluorescence-Activated,Cell Sortings, Fluorescence-Activated,Cytofluorometries, Flow,Cytometries, Flow,Flow Cytofluorometries,Flow Cytofluorometry,Flow Cytometries,Flow Microfluorometries,Flow Microfluorometry,Fluorescence Activated Cell Sorting,Fluorescence-Activated Cell Sortings,Microfluorimetry, Flow,Microfluorometries, Flow,Sorting, Fluorescence-Activated Cell,Sortings, Fluorescence-Activated Cell
D005753 Gastric Mucosa Lining of the STOMACH, consisting of an inner EPITHELIUM, a middle LAMINA PROPRIA, and an outer MUSCULARIS MUCOSAE. The surface cells produce MUCUS that protects the stomach from attack by digestive acid and enzymes. When the epithelium invaginates into the LAMINA PROPRIA at various region of the stomach (CARDIA; GASTRIC FUNDUS; and PYLORUS), different tubular gastric glands are formed. These glands consist of cells that secrete mucus, enzymes, HYDROCHLORIC ACID, or hormones. Cardiac Glands,Gastric Glands,Pyloric Glands,Cardiac Gland,Gastric Gland,Gastric Mucosas,Gland, Cardiac,Gland, Gastric,Gland, Pyloric,Glands, Cardiac,Glands, Gastric,Glands, Pyloric,Mucosa, Gastric,Mucosas, Gastric,Pyloric Gland
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000097802 CD83 Antigen A glycoprotein member of the immunoglobulin superfamily that is primarily expressed on the surface of DENDRITIC CELLS. It is believed to play a role in ANTIGEN PRESENTATION and CELL-TO-CELL INTERACTION following LYMPHOCYTE ACTIVATION. Antigen, CD83,Cell Surface Protein HB15
D015703 Antigens, CD Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation. CD Antigen,Cluster of Differentiation Antigen,Cluster of Differentiation Marker,Differentiation Antigens, Leukocyte, Human,Leukocyte Differentiation Antigens, Human,Cluster of Differentiation Antigens,Cluster of Differentiation Markers,Antigen Cluster, Differentiation,Antigen, CD,CD Antigens,Differentiation Antigen Cluster,Differentiation Marker Cluster,Marker Cluster, Differentiation
D016480 Helicobacter pylori A spiral bacterium active as a human gastric pathogen. It is a gram-negative, urease-positive, curved or slightly spiral organism initially isolated in 1982 from patients with lesions of gastritis or peptic ulcers in Western Australia. Helicobacter pylori was originally classified in the genus CAMPYLOBACTER, but RNA sequencing, cellular fatty acid profiles, growth patterns, and other taxonomic characteristics indicate that the micro-organism should be included in the genus HELICOBACTER. It has been officially transferred to Helicobacter gen. nov. (see Int J Syst Bacteriol 1989 Oct;39(4):297-405). Campylobacter pylori,Campylobacter pylori subsp. pylori,Campylobacter pyloridis,Helicobacter nemestrinae

Related Publications

Anna Helmin-Basa, and Małgorzata Wiese-Szadkowska, and Anna Szaflarska-Popławska, and Maciej Kłosowski, and Milena Januszewska, and Magdalena Bodnar, and Andrzej Marszałek, and Lidia Gackowska, and Jacek Michalkiewicz
January 2004, Journal of pediatric gastroenterology and nutrition,
Anna Helmin-Basa, and Małgorzata Wiese-Szadkowska, and Anna Szaflarska-Popławska, and Maciej Kłosowski, and Milena Januszewska, and Magdalena Bodnar, and Andrzej Marszałek, and Lidia Gackowska, and Jacek Michalkiewicz
November 2013, Klinische Padiatrie,
Anna Helmin-Basa, and Małgorzata Wiese-Szadkowska, and Anna Szaflarska-Popławska, and Maciej Kłosowski, and Milena Januszewska, and Magdalena Bodnar, and Andrzej Marszałek, and Lidia Gackowska, and Jacek Michalkiewicz
October 2002, American journal of respiratory and critical care medicine,
Anna Helmin-Basa, and Małgorzata Wiese-Szadkowska, and Anna Szaflarska-Popławska, and Maciej Kłosowski, and Milena Januszewska, and Magdalena Bodnar, and Andrzej Marszałek, and Lidia Gackowska, and Jacek Michalkiewicz
January 2009, Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases,
Anna Helmin-Basa, and Małgorzata Wiese-Szadkowska, and Anna Szaflarska-Popławska, and Maciej Kłosowski, and Milena Januszewska, and Magdalena Bodnar, and Andrzej Marszałek, and Lidia Gackowska, and Jacek Michalkiewicz
March 2004, Acta paediatrica (Oslo, Norway : 1992),
Anna Helmin-Basa, and Małgorzata Wiese-Szadkowska, and Anna Szaflarska-Popławska, and Maciej Kłosowski, and Milena Januszewska, and Magdalena Bodnar, and Andrzej Marszałek, and Lidia Gackowska, and Jacek Michalkiewicz
March 1991, The American journal of gastroenterology,
Anna Helmin-Basa, and Małgorzata Wiese-Szadkowska, and Anna Szaflarska-Popławska, and Maciej Kłosowski, and Milena Januszewska, and Magdalena Bodnar, and Andrzej Marszałek, and Lidia Gackowska, and Jacek Michalkiewicz
January 2014, Internal medicine (Tokyo, Japan),
Anna Helmin-Basa, and Małgorzata Wiese-Szadkowska, and Anna Szaflarska-Popławska, and Maciej Kłosowski, and Milena Januszewska, and Magdalena Bodnar, and Andrzej Marszałek, and Lidia Gackowska, and Jacek Michalkiewicz
June 2012, Pediatrics international : official journal of the Japan Pediatric Society,
Anna Helmin-Basa, and Małgorzata Wiese-Szadkowska, and Anna Szaflarska-Popławska, and Maciej Kłosowski, and Milena Januszewska, and Magdalena Bodnar, and Andrzej Marszałek, and Lidia Gackowska, and Jacek Michalkiewicz
July 1997, Helicobacter,
Anna Helmin-Basa, and Małgorzata Wiese-Szadkowska, and Anna Szaflarska-Popławska, and Maciej Kłosowski, and Milena Januszewska, and Magdalena Bodnar, and Andrzej Marszałek, and Lidia Gackowska, and Jacek Michalkiewicz
June 2000, Polski merkuriusz lekarski : organ Polskiego Towarzystwa Lekarskiego,
Copied contents to your clipboard!