Biomaterial Database

Enzymes of steroid hormone metabolism in ovarian tissue of teleosts. 1979

B Eckstein, and R Azoury

Institute of Life Sciences, Hebrew University, Jerusalem, Israel.

1. Substrate-velocity kinetics and some other characteristics of three enzymes involved in steroid hormone metabolism in the ovary of the common carp, Cyprinus carpio and of the grey mullet, Mugil cephalus were determined. 2. Labeled steroid products of the enzyme reactions were identified by thin-layer and gas-liquid chromatographies, as well as by formation of derivatives. 3. 5 alpha-Steroid reductase of carp ovary was found to have a Michaelis-Menten constant undistinguishable from that of rat ovarian tissue, while the Km of the enzyme in mullet ovarian tissue was found to be different. 4. The Km of 20 alpha-hydroxysteroid dehydrogenase of mullet ovarian tissue was similar to the value reported for mammalian gonadal tissue, but the constant for 17 beta-hydroxy-steroid dehydrogenase in the carp ovary was different. 5. It is for the first time that these enzymes of steroid hormone metabolism have been quantitatively determined in teleost ovarian tissue.

UI	MeSH Term	Description	Entries
D010053	Ovary	The reproductive organ (GONADS) in female animals. In vertebrates, the ovary contains two functional parts: the OVARIAN FOLLICLE for the production of female germ cells (OOGENESIS); and the endocrine cells (GRANULOSA CELLS; THECA CELLS; and LUTEAL CELLS) for the production of ESTROGENS and PROGESTERONE.	Ovaries
D002347	Carps	Common name for a number of different species of fish in the family Cyprinidae. This includes, among others, the common carp, crucian carp, grass carp, and silver carp.	Carassius carassius,Crucian Carp,Cyprinus,Grass Carp,Carp,Ctenopharyngodon idellus,Cyprinus carpio,Hypophthalmichthys molitrix,Koi Carp,Silver Carp,Carp, Crucian,Carp, Grass,Carp, Koi,Carp, Silver,Carps, Crucian,Carps, Grass,Carps, Silver,Crucian Carps,Grass Carps,Silver Carps
D002849	Chromatography, Gas	Fractionation of a vaporized sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix.	Chromatography, Gas-Liquid,Gas Chromatography,Chromatographies, Gas,Chromatographies, Gas-Liquid,Chromatography, Gas Liquid,Gas Chromatographies,Gas-Liquid Chromatographies,Gas-Liquid Chromatography
D002855	Chromatography, Thin Layer	Chromatography on thin layers of adsorbents rather than in columns. The adsorbent can be alumina, silica gel, silicates, charcoals, or cellulose. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)	Chromatography, Thin-Layer,Thin Layer Chromatography,Chromatographies, Thin Layer,Chromatographies, Thin-Layer,Thin Layer Chromatographies,Thin-Layer Chromatographies,Thin-Layer Chromatography
D005260	Female		Females
D005399	Fishes	A group of cold-blooded, aquatic vertebrates having gills, fins, a cartilaginous or bony endoskeleton, and elongated bodies covered with scales.
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013256	Steroids	A group of polycyclic compounds closely related biochemically to TERPENES. They include cholesterol, numerous hormones, precursors of certain vitamins, bile acids, alcohols (STEROLS), and certain natural drugs and poisons. Steroids have a common nucleus, a fused, reduced 17-carbon atom ring system, cyclopentanoperhydrophenanthrene. Most steroids also have two methyl groups and an aliphatic side-chain attached to the nucleus. (From Hawley's Condensed Chemical Dictionary, 11th ed)	Steroid,Catatoxic Steroids,Steroids, Catatoxic
D013741	3-Oxo-5-alpha-Steroid 4-Dehydrogenase	An enzyme that catalyzes the reduction of TESTOSTERONE to 5-ALPHA DIHYDROTESTOSTERONE.	3-Keto-5-alpha-Steroid delta-4-Dehydrogenase,5-alpha-Reductase, Testosterone,Testosterone 5-alpha-Reductase,Testosterone delta-4-5-alpha-Reductase,3-oxo-5 alpha-Steroid 4-Dehydrogenase (NADP+),3-oxo-5alpha-Steroid 4-Dehydrogenase (NADP+),4-Ene-3-Oxosteroid-5alpha-Reductase,4-Ene-5-alpha-Reductase,NADH-5-alpha-Reductase,NADPH delta(4)-3-Ketosteroid 5 alpha-Oxidoreductase,Steroid 5-alpha-Reductase,Steroid 5Alpha Reductase,Steroid delta-4-5-alpha Reductase,delta 4-3-keto Steroid 5 alpha-Oxidoreductase,delta(4)-3-Ketosteroid-5alpha-Reductase,delta4-3-ketosteroid 5alpha-Oxidoreductase,3 Keto 5 alpha Steroid delta 4 Dehydrogenase,3 Oxo 5 alpha Steroid 4 Dehydrogenase,4 Ene 3 Oxosteroid 5alpha Reductase,4 Ene 5 alpha Reductase,5 alpha Reductase, Testosterone,5-alpha-Reductase, Steroid,5Alpha Reductase, Steroid,5alpha-Oxidoreductase, delta4-3-ketosteroid,NADH 5 alpha Reductase,Reductase, Steroid 5Alpha,Reductase, Steroid delta-4-5-alpha,Steroid 5 alpha Reductase,Steroid delta 4 5 alpha Reductase,Testosterone 5 alpha Reductase,Testosterone delta 4 5 alpha Reductase,delta 4 3 keto Steroid 5 alpha Oxidoreductase,delta-4-5-alpha Reductase, Steroid,delta-4-5-alpha-Reductase, Testosterone,delta-4-Dehydrogenase, 3-Keto-5-alpha-Steroid,delta4 3 ketosteroid 5alpha Oxidoreductase
D015067	17-Hydroxysteroid Dehydrogenases	A class of enzymes that catalyzes the oxidation of 17-hydroxysteroids to 17-ketosteroids. EC 1.1.-.	17 beta-Hydroxysteroid Dehydrogenases,Testosterone Dehydrogenases,17-Ketosteroid Reductases,17 Hydroxysteroid Dehydrogenases,17 Ketosteroid Reductases,17 beta Hydroxysteroid Dehydrogenases,Dehydrogenases, 17 beta-Hydroxysteroid,Dehydrogenases, 17-Hydroxysteroid,Dehydrogenases, Testosterone,Reductases, 17-Ketosteroid