-transparent.png){kind=logo}
An enzyme-labelled immunoassay for plasma cortisol was developed. For this alkaline-phosphatase was conjugated through 21-hemisuccinate of cortisol using water-soluble carbodiimide. An antibody was raised in rabbits against corisol-21-hemisuccinate bovine serum albumin. Before assay 10 mul samples of plasma were mixed with glutamate buffer, pH 3.3, and boiled to denature endogenous cortisol-binding globulin and alkaline-phosphatase. Separation of "bound and free" cortisol was done by the double antibody method. A linear relationship was obtained between the volume of plasma and the amount of cortisol. The minimal detectable level of plasma cortisol was 1 mug/dl and the coefficients of variation were 2.7%-4.4% (within assays) and 4.7%-6.0% (between assays). Cortisol values determined by the present method correlated well with those determined by radioimmunoassay. The present method of enzyme-labelled immunoassay is suitable for routine clinical analysis of plasma cortisol.
T Ogihara, and
K Miyal, and
K Nishi, and
K Ishibashi, and
Y Kumahara
May 1977,
Nihon Naibunpi Gakkai zasshi,
T Ogihara, and
K Miyal, and
K Nishi, and
K Ishibashi, and
Y Kumahara
May 1980,
Clinica chimica acta; international journal of clinical chemistry,
T Ogihara, and
K Miyal, and
K Nishi, and
K Ishibashi, and
Y Kumahara
February 1980,
The Journal of clinical endocrinology and metabolism,
T Ogihara, and
K Miyal, and
K Nishi, and
K Ishibashi, and
Y Kumahara
December 1981,
Chemical & pharmaceutical bulletin,
T Ogihara, and
K Miyal, and
K Nishi, and
K Ishibashi, and
Y Kumahara
June 1992,
Clinica chimica acta; international journal of clinical chemistry,
T Ogihara, and
K Miyal, and
K Nishi, and
K Ishibashi, and
Y Kumahara
July 1984,
Experimental and clinical endocrinology,
T Ogihara, and
K Miyal, and
K Nishi, and
K Ishibashi, and
Y Kumahara
July 1981,
Steroids,
T Ogihara, and
K Miyal, and
K Nishi, and
K Ishibashi, and
Y Kumahara
January 2003,
Clinical chemistry,
T Ogihara, and
K Miyal, and
K Nishi, and
K Ishibashi, and
Y Kumahara
June 1982,
Horumon to rinsho. Clinical endocrinology,
T Ogihara, and
K Miyal, and
K Nishi, and
K Ishibashi, and
Y Kumahara
January 1978,
Steroids,
