A DnaJ protein that interacts with soybean mosaic virus coat protein serves as a key susceptibility factor for viral infection. 2020

Tingxuan Zong, and Jinlong Yin, and Tongtong Jin, and Liqun Wang, and Minxuan Luo, and Kai Li, and Haijian Zhi
National Center for Soybean Improvement, National Key Laboratory for Crop Genetics and Germplasm Enhancement, Key Laboratory of Biology and Genetic Improvement of Soybean, Ministry of Agriculture, Nanjing Agricultural University, Weigang 1, Nanjing, China.

Soybean mosaic virus (SMV)-disease is one of the most serious and widespread diseases in soybean (Glycine max). In the present study, a DnaJ protein in soybean designated GmCPIP (SMV coat protein-interacting protein) was screened by the QIS-Seq (quantitative interactor screening with next-generation sequencing) method, and the interaction between SMV CP and GmCPIP was confirmed by the yeast two-hybrid (Y2H) system and bimolecular fluorescence complementation (BiFC) assay. Subcellular localization analysis indicated that both proteins are localized in the cytoplasm, cytomembrane and nucleus. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis showed that infection with SMV-SC4 temporarily increased the transcription of GmCPIP. Virus-induced gene silencing (VIGS) down-regulated the GmCPIP gene by 82%, and the accumulation of SMV was decreased by 88.6% in GmCPIP-silenced plants inoculated with SMV-SC4. The interaction of GmCPIP with SMV CP seems to contribute to SMV infection in soybean.

UI MeSH Term Description Entries
D010935 Plant Diseases Diseases of plants. Disease, Plant,Diseases, Plant,Plant Disease
D010940 Plant Proteins Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which PLANT PROTEINS, DIETARY is available. Plant Protein,Protein, Plant,Proteins, Plant
D011485 Protein Binding The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments. Plasma Protein Binding Capacity,Binding, Protein
D000076662 Host Microbial Interactions Interactions between a host and microbe or microbiota. Host-Bacteria Interactions,Host-Microbe Interactions,Host-Microbial Interactions,Host-Virus Interactions,Microbe-Host Interactions,Microbial-Host Interactions,Microbiota-Host Interactions,Virus-Host Interactions,Bacteria Host Interactions,Bacterial-Host Interactions,Bacterium-Host Interactions,Host Bacteria Interactions,Host Microbe Interactions,Host Microbiota Interactions,Host Virus Interactions,Host-Fungal Interactions,Host-Microbial Interface,Microbe Host Interactions,Microbial Host Interactions,Microbiota Host Interactions,Viral-Host Interactions,Virus Host Interactions,Bacteria Host Interaction,Bacterial Host Interactions,Bacterial-Host Interaction,Bacterium Host Interactions,Bacterium-Host Interaction,Host Bacteria Interaction,Host Fungal Interactions,Host Microbe Interaction,Host Microbial Interaction,Host Microbial Interface,Host Microbiota Interaction,Host Virus Interaction,Host-Bacteria Interaction,Host-Fungal Interaction,Host-Microbe Interaction,Host-Microbial Interaction,Host-Microbial Interfaces,Host-Virus Interaction,Interaction, Host-Bacteria,Interaction, Host-Microbe,Interaction, Host-Microbial,Interaction, Host-Virus,Interaction, Microbe-Host,Interaction, Microbial-Host,Interaction, Microbiota-Host,Interaction, Virus-Host,Interactions, Host-Bacteria,Interactions, Host-Microbe,Interactions, Host-Microbial,Interactions, Host-Virus,Interactions, Microbe-Host,Interactions, Microbial-Host,Interactions, Microbiota-Host,Interactions, Virus-Host,Microbe Host Interaction,Microbe-Host Interaction,Microbial Host Interaction,Microbial-Host Interaction,Microbiota Host Interaction,Microbiota-Host Interaction,Viral Host Interactions,Viral-Host Interaction,Virus Host Interaction,Virus-Host Interaction
D013025 Glycine max An annual legume. The SEEDS of this plant are edible and used to produce a variety of SOY FOODS. Soy Beans,Soybeans,Bean, Soy,Beans, Soy,Soy Bean,Soybean
D014777 Virus Diseases A general term for diseases caused by viruses. Viral Diseases,Viral Infections,Virus Infections,Disease, Viral,Disease, Virus,Diseases, Viral,Diseases, Virus,Infection, Viral,Infection, Virus,Infections, Viral,Infections, Virus,Viral Disease,Viral Infection,Virus Disease,Virus Infection
D050956 HSP40 Heat-Shock Proteins A family of heat-shock proteins that contain a 70 amino-acid consensus sequence known as the J domain. The J domain of HSP40 heat shock proteins interacts with HSP70 HEAT-SHOCK PROTEINS. HSP40 heat-shock proteins play a role in regulating the ADENOSINE TRIPHOSPHATASES activity of HSP70 heat-shock proteins. DnaJ Protein,HSP40 Heat-Shock Protein,HSP40 Protein,Heat-Shock Proteins 40,DnaJ Proteins,HSP40 Protein Family,HSP40 Proteins,Heat Shock Protein 40 Family,Heat-Shock Protein 40,HSP40 Heat Shock Protein,HSP40 Heat Shock Proteins,Heat Shock Protein 40,Heat Shock Proteins 40,Heat-Shock Protein, HSP40,Heat-Shock Proteins, HSP40,Protein, DnaJ,Protein, HSP40,Protein, HSP40 Heat-Shock,Proteins, HSP40 Heat-Shock
D017800 Potyvirus A large genus of plant viruses of the family POTYVIRIDAE which infect mainly plants of the Solanaceae. Transmission is primarily by aphids in a non-persistent manner. The type species is potato virus Y. Potato Virus Y,Potato Virus Ys,Potyviruses,Virus Ys, Potato,Ys, Potato Virus
D036022 Capsid Proteins Proteins that form the CAPSID of VIRUSES. Procapsid Protein,Procapsid Proteins,Viral Coat Protein,Viral Coat Proteins,Viral V Antigens,Viral V Proteins,Capsid Protein,Viral Outer Coat Protein,Antigens, Viral V,Coat Protein, Viral,V Antigens, Viral,V Proteins, Viral

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