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Inhibitors of sterol synthesis. 14 alpha-ethyl-5 alpha-cholest-7-ene-3 beta,15 alpha-diol induces changes in the sterol composition and the morphology of CHO-K1 cells. 1988

F D Pinkerton, and A Izumi, and G J Schroepfer
Department of Biochemistry, Rice University, Houston, Texas 77251.

Incubation of CHO-K1 cells in lipid-deficient medium containing 14 alpha-ethyl-5 alpha-cholest-7-ene-3 beta,15 alpha-diol (0.1 microM) for 4 days was associated with a profound change in cellular sterol composition as reflected by a marked accumulation of lanosterol and 24,25-dihydrolanosterol. A striking elongation of the cells was also observed. Incubation of CHO-K1 cells in lipid-deficient medium containing lanosterol (10 microM) also caused a significant accumulation of lanosterol which was also associated with a marked elongation of the cells.

UI MeSH Term Description Entries
D007810 Lanosterol A triterpene that derives from the chair-boat-chair-boat folding of 2,3-oxidosqualene. It is metabolized to CHOLESTEROL and CUCURBITACINS. Kryptosterol,4,4,14 alpha-trimethyl-5 alpha-cholesta-8,24-dien-3 beta-ol
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D002782 Cholestenes Steroids with methyl groups at C-10 and C-13 and a branched 8-carbon chain at C-17. Members include compounds with any degree of unsaturation; however, CHOLESTADIENES is available for derivatives containing two double bonds.
D002784 Cholesterol The principal sterol of all higher animals, distributed in body tissues, especially the brain and spinal cord, and in animal fats and oils. Epicholesterol
D006224 Cricetinae A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS. Cricetus,Hamsters,Hamster
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013261 Sterols Steroids with a hydroxyl group at C-3 and most of the skeleton of cholestane. Additional carbon atoms may be present in the side chain. (IUPAC Steroid Nomenclature, 1987) Sterol

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