-transparent.png){kind=logo}
Long-gap peripheral nerve repair through sustained release of a neurotrophic factor in nonhuman primates. 2020
Neil B Fadia, and
Jacqueline M Bliley, and
Gabriella A DiBernardo, and
Donald J Crammond, and
Benjamin K Schilling, and
Wesley N Sivak, and
Alexander M Spiess, and
Kia M Washington, and
Matthias Waldner, and
Han-Tsung Liao, and
Isaac B James, and
Danielle M Minteer, and
Casey Tompkins-Rhoades, and
Adam R Cottrill, and
Deok-Yeol Kim, and
Riccardo Schweizer, and
Debra A Bourne, and
George E Panagis, and
M Asher Schusterman, and
Francesco M Egro, and
Insiyah K Campwala, and
Tyler Simpson, and
Douglas J Weber, and
Trent Gause, and
Jack E Brooker, and
Tvisha Josyula, and
Astrid A Guevara, and
Alexander J Repko, and
Christopher M Mahoney, and
Kacey G Marra
Department of Plastic Surgery, University of Pittsburgh, Pittsburgh, PA 15213, USA.
Severe injuries to peripheral nerves are challenging to repair. Standard-of-care treatment for nerve gaps >2 to 3 centimeters is autografting; however, autografting can result in neuroma formation, loss of sensory function at the donor site, and increased operative time. To address the need for a synthetic nerve conduit to treat large nerve gaps, we investigated a biodegradable poly(caprolactone) (PCL) conduit with embedded double-walled polymeric microspheres encapsulating glial cell line-derived neurotrophic factor (GDNF) capable of providing a sustained release of GDNF for >50 days in a 5-centimeter nerve defect in a rhesus macaque model. The GDNF-eluting conduit (PCL/GDNF) was compared to a median nerve autograft and a PCL conduit containing empty microspheres (PCL/Empty). Functional testing demonstrated similar functional recovery between the PCL/GDNF-treated group (75.64 ± 10.28%) and the autograft-treated group (77.49 ± 19.28%); both groups were statistically improved compared to PCL/Empty-treated group (44.95 ± 26.94%). Nerve conduction velocity 1 year after surgery was increased in the PCL/GDNF-treated macaques (31.41 ± 15.34 meters/second) compared to autograft (25.45 ± 3.96 meters/second) and PCL/Empty (12.60 ± 3.89 meters/second) treatment. Histological analyses included assessment of Schwann cell presence, myelination of axons, nerve fiber density, and g-ratio. PCL/GDNF group exhibited a statistically greater average area occupied by individual Schwann cells at the distal nerve (11.60 ± 33.01 μm2) compared to autograft (4.62 ± 3.99 μm2) and PCL/Empty (4.52 ± 5.16 μm2) treatment groups. This study demonstrates the efficacious bridging of a long peripheral nerve gap in a nonhuman primate model using an acellular, biodegradable nerve conduit.
Related Publications
Neil B Fadia, and
Jacqueline M Bliley, and
Gabriella A DiBernardo, and
Donald J Crammond, and
Benjamin K Schilling, and
Wesley N Sivak, and
Alexander M Spiess, and
Kia M Washington, and
Matthias Waldner, and
Han-Tsung Liao, and
Isaac B James, and
Danielle M Minteer, and
Casey Tompkins-Rhoades, and
Adam R Cottrill, and
Deok-Yeol Kim, and
Riccardo Schweizer, and
Debra A Bourne, and
George E Panagis, and
M Asher Schusterman, and
Francesco M Egro, and
Insiyah K Campwala, and
Tyler Simpson, and
Douglas J Weber, and
Trent Gause, and
Jack E Brooker, and
Tvisha Josyula, and
Astrid A Guevara, and
Alexander J Repko, and
Christopher M Mahoney, and
Kacey G Marra
May 2011,
Tissue engineering. Part A,
Neil B Fadia, and
Jacqueline M Bliley, and
Gabriella A DiBernardo, and
Donald J Crammond, and
Benjamin K Schilling, and
Wesley N Sivak, and
Alexander M Spiess, and
Kia M Washington, and
Matthias Waldner, and
Han-Tsung Liao, and
Isaac B James, and
Danielle M Minteer, and
Casey Tompkins-Rhoades, and
Adam R Cottrill, and
Deok-Yeol Kim, and
Riccardo Schweizer, and
Debra A Bourne, and
George E Panagis, and
M Asher Schusterman, and
Francesco M Egro, and
Insiyah K Campwala, and
Tyler Simpson, and
Douglas J Weber, and
Trent Gause, and
Jack E Brooker, and
Tvisha Josyula, and
Astrid A Guevara, and
Alexander J Repko, and
Christopher M Mahoney, and
Kacey G Marra
January 2014,
American journal of translational research,
Neil B Fadia, and
Jacqueline M Bliley, and
Gabriella A DiBernardo, and
Donald J Crammond, and
Benjamin K Schilling, and
Wesley N Sivak, and
Alexander M Spiess, and
Kia M Washington, and
Matthias Waldner, and
Han-Tsung Liao, and
Isaac B James, and
Danielle M Minteer, and
Casey Tompkins-Rhoades, and
Adam R Cottrill, and
Deok-Yeol Kim, and
Riccardo Schweizer, and
Debra A Bourne, and
George E Panagis, and
M Asher Schusterman, and
Francesco M Egro, and
Insiyah K Campwala, and
Tyler Simpson, and
Douglas J Weber, and
Trent Gause, and
Jack E Brooker, and
Tvisha Josyula, and
Astrid A Guevara, and
Alexander J Repko, and
Christopher M Mahoney, and
Kacey G Marra
May 1980,
The Journal of hand surgery,
Neil B Fadia, and
Jacqueline M Bliley, and
Gabriella A DiBernardo, and
Donald J Crammond, and
Benjamin K Schilling, and
Wesley N Sivak, and
Alexander M Spiess, and
Kia M Washington, and
Matthias Waldner, and
Han-Tsung Liao, and
Isaac B James, and
Danielle M Minteer, and
Casey Tompkins-Rhoades, and
Adam R Cottrill, and
Deok-Yeol Kim, and
Riccardo Schweizer, and
Debra A Bourne, and
George E Panagis, and
M Asher Schusterman, and
Francesco M Egro, and
Insiyah K Campwala, and
Tyler Simpson, and
Douglas J Weber, and
Trent Gause, and
Jack E Brooker, and
Tvisha Josyula, and
Astrid A Guevara, and
Alexander J Repko, and
Christopher M Mahoney, and
Kacey G Marra
July 2015,
ACS biomaterials science & engineering,
Neil B Fadia, and
Jacqueline M Bliley, and
Gabriella A DiBernardo, and
Donald J Crammond, and
Benjamin K Schilling, and
Wesley N Sivak, and
Alexander M Spiess, and
Kia M Washington, and
Matthias Waldner, and
Han-Tsung Liao, and
Isaac B James, and
Danielle M Minteer, and
Casey Tompkins-Rhoades, and
Adam R Cottrill, and
Deok-Yeol Kim, and
Riccardo Schweizer, and
Debra A Bourne, and
George E Panagis, and
M Asher Schusterman, and
Francesco M Egro, and
Insiyah K Campwala, and
Tyler Simpson, and
Douglas J Weber, and
Trent Gause, and
Jack E Brooker, and
Tvisha Josyula, and
Astrid A Guevara, and
Alexander J Repko, and
Christopher M Mahoney, and
Kacey G Marra
January 2004,
Drug delivery,
Neil B Fadia, and
Jacqueline M Bliley, and
Gabriella A DiBernardo, and
Donald J Crammond, and
Benjamin K Schilling, and
Wesley N Sivak, and
Alexander M Spiess, and
Kia M Washington, and
Matthias Waldner, and
Han-Tsung Liao, and
Isaac B James, and
Danielle M Minteer, and
Casey Tompkins-Rhoades, and
Adam R Cottrill, and
Deok-Yeol Kim, and
Riccardo Schweizer, and
Debra A Bourne, and
George E Panagis, and
M Asher Schusterman, and
Francesco M Egro, and
Insiyah K Campwala, and
Tyler Simpson, and
Douglas J Weber, and
Trent Gause, and
Jack E Brooker, and
Tvisha Josyula, and
Astrid A Guevara, and
Alexander J Repko, and
Christopher M Mahoney, and
Kacey G Marra
January 2023,
Neural regeneration research,
Neil B Fadia, and
Jacqueline M Bliley, and
Gabriella A DiBernardo, and
Donald J Crammond, and
Benjamin K Schilling, and
Wesley N Sivak, and
Alexander M Spiess, and
Kia M Washington, and
Matthias Waldner, and
Han-Tsung Liao, and
Isaac B James, and
Danielle M Minteer, and
Casey Tompkins-Rhoades, and
Adam R Cottrill, and
Deok-Yeol Kim, and
Riccardo Schweizer, and
Debra A Bourne, and
George E Panagis, and
M Asher Schusterman, and
Francesco M Egro, and
Insiyah K Campwala, and
Tyler Simpson, and
Douglas J Weber, and
Trent Gause, and
Jack E Brooker, and
Tvisha Josyula, and
Astrid A Guevara, and
Alexander J Repko, and
Christopher M Mahoney, and
Kacey G Marra
April 2024,
Journal of biomaterials science. Polymer edition,
Neil B Fadia, and
Jacqueline M Bliley, and
Gabriella A DiBernardo, and
Donald J Crammond, and
Benjamin K Schilling, and
Wesley N Sivak, and
Alexander M Spiess, and
Kia M Washington, and
Matthias Waldner, and
Han-Tsung Liao, and
Isaac B James, and
Danielle M Minteer, and
Casey Tompkins-Rhoades, and
Adam R Cottrill, and
Deok-Yeol Kim, and
Riccardo Schweizer, and
Debra A Bourne, and
George E Panagis, and
M Asher Schusterman, and
Francesco M Egro, and
Insiyah K Campwala, and
Tyler Simpson, and
Douglas J Weber, and
Trent Gause, and
Jack E Brooker, and
Tvisha Josyula, and
Astrid A Guevara, and
Alexander J Repko, and
Christopher M Mahoney, and
Kacey G Marra
April 1991,
The Journal of comparative neurology,
Neil B Fadia, and
Jacqueline M Bliley, and
Gabriella A DiBernardo, and
Donald J Crammond, and
Benjamin K Schilling, and
Wesley N Sivak, and
Alexander M Spiess, and
Kia M Washington, and
Matthias Waldner, and
Han-Tsung Liao, and
Isaac B James, and
Danielle M Minteer, and
Casey Tompkins-Rhoades, and
Adam R Cottrill, and
Deok-Yeol Kim, and
Riccardo Schweizer, and
Debra A Bourne, and
George E Panagis, and
M Asher Schusterman, and
Francesco M Egro, and
Insiyah K Campwala, and
Tyler Simpson, and
Douglas J Weber, and
Trent Gause, and
Jack E Brooker, and
Tvisha Josyula, and
Astrid A Guevara, and
Alexander J Repko, and
Christopher M Mahoney, and
Kacey G Marra
March 2022,
Nanomedicine (London, England),
Neil B Fadia, and
Jacqueline M Bliley, and
Gabriella A DiBernardo, and
Donald J Crammond, and
Benjamin K Schilling, and
Wesley N Sivak, and
Alexander M Spiess, and
Kia M Washington, and
Matthias Waldner, and
Han-Tsung Liao, and
Isaac B James, and
Danielle M Minteer, and
Casey Tompkins-Rhoades, and
Adam R Cottrill, and
Deok-Yeol Kim, and
Riccardo Schweizer, and
Debra A Bourne, and
George E Panagis, and
M Asher Schusterman, and
Francesco M Egro, and
Insiyah K Campwala, and
Tyler Simpson, and
Douglas J Weber, and
Trent Gause, and
Jack E Brooker, and
Tvisha Josyula, and
Astrid A Guevara, and
Alexander J Repko, and
Christopher M Mahoney, and
Kacey G Marra
May 2024,
Acta biomaterialia,
