Biomaterial Database

Synergistic effect of hydroxyurea and excessive thymidine on the expression of the common fragile sites at 3p14 and 16q23. 1988

Z A Yan, and X Z Li, and X T Zhou

Institute of Genetics, Academia Sinica, Beijing, China.

There is a synergistic effect between hydroxyurea (HU) and high concentrations of thymidine (TdR) on the induction of the common fragile sites at 3p14 and 16q23. The frequencies of expression of these fragile sites were significantly higher than in the controls when excessive TdR (0.5 mM) and HU (4 mM) were added into lymphocyte cultures in MEM medium 24 h and 2 h before harvest. Two conditions for the maximum expression of common fragile sites, such as fra(3)(p14), fra(16)(q23) are discussed. The TdR-HU fra(3)(p14)-inducing system might be modified effectively and consistently to improve the prenatal diagnosis of the fragile X syndrome.

UI	MeSH Term	Description	Entries
D002873	Chromosome Fragility	Susceptibility of chromosomes to breakage leading to translocation; CHROMOSOME INVERSION; SEQUENCE DELETION; or other CHROMOSOME BREAKAGE related aberrations.	Chromosomal Fragility,Fragility, Chromosomal,Fragility, Chromosome
D002885	Chromosomes, Human, Pair 16	A specific pair of GROUP E CHROMOSOMES of the human chromosome classification.	Chromosome 16
D002893	Chromosomes, Human, Pair 3	A specific pair of human chromosomes in group A (CHROMOSOMES, HUMAN, 1-3) of the human chromosome classification.	Chromosome 3
D004357	Drug Synergism	The action of a drug in promoting or enhancing the effectiveness of another drug.	Drug Potentiation,Drug Augmentation,Augmentation, Drug,Augmentations, Drug,Drug Augmentations,Drug Potentiations,Drug Synergisms,Potentiation, Drug,Potentiations, Drug,Synergism, Drug,Synergisms, Drug
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006918	Hydroxyurea	An antineoplastic agent that inhibits DNA synthesis through the inhibition of ribonucleoside diphosphate reductase.	Hydroxycarbamid,Hydrea,Oncocarbide
D013936	Thymidine	A nucleoside in which THYMINE is linked to DEOXYRIBOSE.	2'-Deoxythymidine,Deoxythymidine,2' Deoxythymidine
D043283	Chromosome Fragile Sites	Specific loci that show up during KARYOTYPING as a gap (an uncondensed stretch in closer views) on a CHROMATID arm after culturing cells under specific conditions. These sites are associated with an increase in CHROMOSOME FRAGILITY. They are classified as common or rare, and by the specific culture conditions under which they develop. Fragile site loci are named by the letters "FRA" followed by a designation for the specific chromosome, and a letter which refers to which fragile site of that chromosome (e.g. FRAXA refers to fragile site A on the X chromosome. It is a rare, folic acid-sensitive fragile site associated with FRAGILE X SYNDROME.)	Fragile Sites, Chromosome,Chromosome Fragile Site,Fragile Site, Chromosome,Site, Chromosome Fragile,Sites, Chromosome Fragile