Biomaterial Database

Urinary excretion of 3-methyladenine in humans as a marker of nucleic acid methylation. 1988

D E Shuker, and P B Farmer

International Agency for Research on Cancer, Lyon, France.

Antisera to 3-methyladenine (3-meAde) were obtained using a novel analogue of 3-meAde which was bound covalently to methylated bovine serum albumin and used as an antigen. 3-meAde keyhole limpet haemocyanin (3-meAde-KLH) was detected at high dilutions of the antisera (1 in 10(4), and 3-meAde itself inhibited the recognition. In an enzyme-linked immunosorbent assay (ELISA), at room temperature, 3-meAde was detected at 1 pmol/well; however, in a non-equilibrium assay at 4 degrees C, a considerable enhancement of sensitivity was obtained, and 3-meAde was detected at 70 fmol/well. Due to the presence of other purines, the direct determination of urinary 3-meAde was not possible, and a high-performance liquid chromatography (HPLC) clean-up step, followed by ELISA, has been developed. Human urine samples have been analysed by this method and the results compared to those obtained by a gas chromatography-mass spectrometric (GC-MS) method.

UI	MeSH Term	Description	Entries
D008745	Methylation	Addition of methyl groups. In histo-chemistry methylation is used to esterify carboxyl groups and remove sulfate groups by treating tissue sections with hot methanol in the presence of hydrochloric acid. (From Stedman, 25th ed)	Methylations
D004247	DNA	A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).	DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D004797	Enzyme-Linked Immunosorbent Assay	An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.	ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000225	Adenine	A purine base and a fundamental unit of ADENINE NUCLEOTIDES.	Vitamin B 4,4, Vitamin B,B 4, Vitamin