BIOMAT Database Logo BIOMAT Database Logo

Anion exchange chromatography of oligonucleotides under denaturing conditions. 2020

Jinyu Sun, and Dhruval Joshi, and Frank Betancourt, and Andrei Solodinin, and Breyer Woodland, and Hongbin Yan
Department of Chemistry, Brock University, St. Catharines, Canada.

Denaturing anion exchange HPLC simplifies the chromatographic profiles of self-complementary sequences such as d(CG)6, d(CG)6 with locked nucleic acid modifications, d(AT)15, and polymerase chain reaction mixtures. These chromatographic conditions use eluents containing up to 4 M urea at pH 12.4, and lead to the abolishment of secondary structures and meaningful chromatographic patterns of self-complementary sequences. Similarly, PCR template, FAM-labelled primer and FAM-labelled PCR products were resolved, making interpretation of PCR reaction products possible.

UI MeSH Term Description Entries
D009690 Nucleic Acid Conformation The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape. DNA Conformation,RNA Conformation,Conformation, DNA,Conformation, Nucleic Acid,Conformation, RNA,Conformations, DNA,Conformations, Nucleic Acid,Conformations, RNA,DNA Conformations,Nucleic Acid Conformations,RNA Conformations
D009691 Nucleic Acid Denaturation Disruption of the secondary structure of nucleic acids by heat, extreme pH or chemical treatment. Double strand DNA is "melted" by dissociation of the non-covalent hydrogen bonds and hydrophobic interactions. Denatured DNA appears to be a single-stranded flexible structure. The effects of denaturation on RNA are similar though less pronounced and largely reversible. DNA Denaturation,DNA Melting,RNA Denaturation,Acid Denaturation, Nucleic,Denaturation, DNA,Denaturation, Nucleic Acid,Denaturation, RNA,Nucleic Acid Denaturations
D009841 Oligonucleotides Polymers made up of a few (2-20) nucleotides. In molecular genetics, they refer to a short sequence synthesized to match a region where a mutation is known to occur, and then used as a probe (OLIGONUCLEOTIDE PROBES). (Dorland, 28th ed) Oligonucleotide
D009994 Osmolar Concentration The concentration of osmotically active particles in solution expressed in terms of osmoles of solute per liter of solution. Osmolality is expressed in terms of osmoles of solute per kilogram of solvent. Ionic Strength,Osmolality,Osmolarity,Concentration, Osmolar,Concentrations, Osmolar,Ionic Strengths,Osmolalities,Osmolar Concentrations,Osmolarities,Strength, Ionic,Strengths, Ionic
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D002852 Chromatography, Ion Exchange Separation technique in which the stationary phase consists of ion exchange resins. The resins contain loosely held small ions that easily exchange places with other small ions of like charge present in solutions washed over the resins. Chromatography, Ion-Exchange,Ion-Exchange Chromatography,Chromatographies, Ion Exchange,Chromatographies, Ion-Exchange,Ion Exchange Chromatographies,Ion Exchange Chromatography,Ion-Exchange Chromatographies
D006863 Hydrogen-Ion Concentration The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D014508 Urea A compound formed in the liver from ammonia produced by the deamination of amino acids. It is the principal end product of protein catabolism and constitutes about one half of the total urinary solids. Basodexan,Carbamide,Carmol
D016133 Polymerase Chain Reaction In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships. Anchored PCR,Inverse PCR,Nested PCR,PCR,Anchored Polymerase Chain Reaction,Inverse Polymerase Chain Reaction,Nested Polymerase Chain Reaction,PCR, Anchored,PCR, Inverse,PCR, Nested,Polymerase Chain Reactions,Reaction, Polymerase Chain,Reactions, Polymerase Chain

Related Publications

Jinyu Sun, and Dhruval Joshi, and Frank Betancourt, and Andrei Solodinin, and Breyer Woodland, and Hongbin Yan
Separation of the diastereomers of phosphorothioated siRNAs by anion-exchange chromatography under non-denaturing conditions.
April 2024, Journal of chromatography. A,
Jinyu Sun, and Dhruval Joshi, and Frank Betancourt, and Andrei Solodinin, and Breyer Woodland, and Hongbin Yan
High-performance anion-exchange chromatography of oligonucleotides.
February 1985, Analytical biochemistry,
Jinyu Sun, and Dhruval Joshi, and Frank Betancourt, and Andrei Solodinin, and Breyer Woodland, and Hongbin Yan
Retention behavior of short double-stranded oligonucleotide and its potential impurities by anion-exchange chromatography under non-denaturing conditions.
February 2023, Journal of chromatography. A,
Jinyu Sun, and Dhruval Joshi, and Frank Betancourt, and Andrei Solodinin, and Breyer Woodland, and Hongbin Yan
Sequence analysis of synthetic oligonucleotides by high-performance liquid anion-exchange chromatography.
January 1977, Journal of chromatography,
Jinyu Sun, and Dhruval Joshi, and Frank Betancourt, and Andrei Solodinin, and Breyer Woodland, and Hongbin Yan
Anion-exchange chromatography of phosphopeptides: weak anion exchange versus strong anion exchange and anion-exchange chromatography versus electrostatic repulsion-hydrophilic interaction chromatography.
January 2015, Analytical chemistry,
Jinyu Sun, and Dhruval Joshi, and Frank Betancourt, and Andrei Solodinin, and Breyer Woodland, and Hongbin Yan
Computer-assisted retention prediction system for oligonucleotides in gradient anion-exchange chromatography.
December 1988, Journal of chromatography,
Jinyu Sun, and Dhruval Joshi, and Frank Betancourt, and Andrei Solodinin, and Breyer Woodland, and Hongbin Yan
Quantitative analysis of phosphorothioate oligonucleotides in biological fluids using fast anion-exchange chromatography.
July 1993, Journal of chromatography,
Jinyu Sun, and Dhruval Joshi, and Frank Betancourt, and Andrei Solodinin, and Breyer Woodland, and Hongbin Yan
Cracking Proteoform Complexity of Ovalbumin with Anion-Exchange Chromatography-High-Resolution Mass Spectrometry under Native Conditions.
October 2019, Journal of proteome research,
Jinyu Sun, and Dhruval Joshi, and Frank Betancourt, and Andrei Solodinin, and Breyer Woodland, and Hongbin Yan
Anion-exchange HPLC analysis of biotinylated oligonucleotides.
August 1995, BioTechniques,
Jinyu Sun, and Dhruval Joshi, and Frank Betancourt, and Andrei Solodinin, and Breyer Woodland, and Hongbin Yan
Isoelectric focusing under denaturing conditions.
January 1988, Methods in molecular biology (Clifton, N.J.),
Copied contents to your clipboard!