OBJECTIVE To determine the relationship between Chlamydia Trachomatis infections with patency tubal and non-patency tubal occurrence in infertile women. METHODS This research was an observational analytic research with a cross-sectional study. This research was conducted at Haji Adam Malik General Hospital Medan, Pramita Laboratory Medan and Medical Faculty of Medicine Universitas Sumatera Utara. Laboratory on October 2017 until the number of samples was fulfilled. The samples were women who were referred to Pramita Laboratory Medan for hysterosalpingography procedure in accordance which corresponded to the inclusion criteria by using consecutive sampling technique. RESULTS From this research, it was found that the most infertile women were aged 31-35 years, with the most infertility type was primary infertility (83,8%) with the longest infertility duration was ≥ 3 years (44%). From all samples who were infertile, 26% among them were positive to chlamydia infection. Eight from twelve people who were infected by Chlamydia Trachomatis experienced non-patency tubal (66.7%) with p-value = 0.001 which showed that there was a relationship between Chlamydia Trachomatis infection with patency tubal and non-patency tubal occurrence in infertile women. CONCLUSIONS The proportion of Chlamydia Trachomatis infection in tubal abnormality in this study was 66.7%, whereas Chlamydia Trachomatis infection in the normal tube was 13.2%. It was obtained that there was a significant relationship between Chlamydia Trachomatis infection with tubal abnormality (non-patency tubal) with p-value < 0.005 (p = 0.001).