RNA-binding as chaperones of DNA binding proteins from starved cells. 2020

Chan Park, and Yoontae Jin, and Young Jun Kim, and Hotcherl Jeong, and Baik L Seong
Department of Biotechnology, Yonsei University, Seoul, 03722, Republic of Korea; Department of Biomaterials Science and Engineering, Yonsei University, Seoul, 03722, Republic of Korea.

DNA-binding proteins from starved cells (Dps) in Escherichia coli protects DNA from multiple stresses during the stationary phase by forming a stable Dps-DNA complex. In contrast, Dps cannot bind to DNA during the exponential phase and it has not been clear why Dps conditionally binds to DNA depending on the growth phase. In this study, we show that DNA-free Dps in the exponential phase can also bind to RNA and the preemptive binding of RNA precludes DNA from interacting with Dps. The critical role of RNA in modulating the stability and functional competence of Dps and their morphology, leads us to propose a two-state model of Dps in executing stress responses. In the exponential phase, Dps is present predominantly as ribonucleoprotein complex. Under starvation, RNAs are degraded by up-regulated RNases, activating Dps to bind with chromosomal DNAs protecting them from diverse stresses. A dual role of RNA as an inhibitor of DNA binding and chaperone to keep dynamic functional status of Dps would be crucial for operating an immediate protection of chromosomal DNAs on starvation. The holdase-type chaperoning role of RNA in Dps-mediated stress responses would shed light on the role of RNAs as chaperone (Chaperna).

UI MeSH Term Description Entries
D004268 DNA-Binding Proteins Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases. DNA Helix Destabilizing Proteins,DNA-Binding Protein,Single-Stranded DNA Binding Proteins,DNA Binding Protein,DNA Single-Stranded Binding Protein,SS DNA BP,Single-Stranded DNA-Binding Protein,Binding Protein, DNA,DNA Binding Proteins,DNA Single Stranded Binding Protein,DNA-Binding Protein, Single-Stranded,Protein, DNA-Binding,Single Stranded DNA Binding Protein,Single Stranded DNA Binding Proteins
D004269 DNA, Bacterial Deoxyribonucleic acid that makes up the genetic material of bacteria. Bacterial DNA
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D004927 Escherichia coli Infections Infections with bacteria of the species ESCHERICHIA COLI. E coli Infections,E. coli Infection,Infections, E coli,Infections, Escherichia coli,E coli Infection,E. coli Infections,Escherichia coli Infection,Infection, E coli,Infection, E. coli,Infection, Escherichia coli
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012329 RNA, Bacterial Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis. Bacterial RNA
D013312 Stress, Physiological The unfavorable effect of environmental factors (stressors) on the physiological functions of an organism. Prolonged unresolved physiological stress can affect HOMEOSTASIS of the organism, and may lead to damaging or pathological conditions. Biotic Stress,Metabolic Stress,Physiological Stress,Abiotic Stress,Abiotic Stress Reaction,Abiotic Stress Response,Biological Stress,Metabolic Stress Response,Physiological Stress Reaction,Physiological Stress Reactivity,Physiological Stress Response,Abiotic Stress Reactions,Abiotic Stress Responses,Abiotic Stresses,Biological Stresses,Biotic Stresses,Metabolic Stress Responses,Metabolic Stresses,Physiological Stress Reactions,Physiological Stress Responses,Physiological Stresses,Reaction, Abiotic Stress,Reactions, Abiotic Stress,Response, Abiotic Stress,Response, Metabolic Stress,Stress Reaction, Physiological,Stress Response, Metabolic,Stress Response, Physiological,Stress, Abiotic,Stress, Biological,Stress, Biotic,Stress, Metabolic
D029968 Escherichia coli Proteins Proteins obtained from ESCHERICHIA COLI. E coli Proteins

Related Publications

Chan Park, and Yoontae Jin, and Young Jun Kim, and Hotcherl Jeong, and Baik L Seong
January 2010, Frontiers in bioscience (Landmark edition),
Chan Park, and Yoontae Jin, and Young Jun Kim, and Hotcherl Jeong, and Baik L Seong
January 2014, Annual review of biochemistry,
Chan Park, and Yoontae Jin, and Young Jun Kim, and Hotcherl Jeong, and Baik L Seong
January 2011, Wiley interdisciplinary reviews. RNA,
Chan Park, and Yoontae Jin, and Young Jun Kim, and Hotcherl Jeong, and Baik L Seong
January 1997, Folding & design,
Chan Park, and Yoontae Jin, and Young Jun Kim, and Hotcherl Jeong, and Baik L Seong
January 2011, Neurochemical research,
Chan Park, and Yoontae Jin, and Young Jun Kim, and Hotcherl Jeong, and Baik L Seong
December 2011, Journal of neuroimmunology,
Chan Park, and Yoontae Jin, and Young Jun Kim, and Hotcherl Jeong, and Baik L Seong
January 2018, Molekuliarnaia biologiia,
Chan Park, and Yoontae Jin, and Young Jun Kim, and Hotcherl Jeong, and Baik L Seong
July 2023, Biochemical and biophysical research communications,
Chan Park, and Yoontae Jin, and Young Jun Kim, and Hotcherl Jeong, and Baik L Seong
February 1975, Biochimica et biophysica acta,
Chan Park, and Yoontae Jin, and Young Jun Kim, and Hotcherl Jeong, and Baik L Seong
February 1987, Biochimica et biophysica acta,
Copied contents to your clipboard!