Biomaterial Database

Identification of the two cell groups in the longitudinal muscle layer of the earthworm Amynthas hawayanus by a single microelectrode with lucifer yellow-CH. 1988

Y C Chang, and Z Assmé

Departamento de Ciências Fisiológicas, Universidade Federal do Paraná, Curitiba, Brasil.

Previous electrophysiological studies have suggested the presence of two types of muscular cells in the longitudinal muscle of A. hawayanus. However, a definite classification of such cells as muscles has been hindered by lack of morphological evidence. This study identified these cells electrophysiologically and morphologically by using the same intracellular microelectrode to record cell activity and inject Lucifer Yellow-CH. Electrophysiological measurements clearly differentiated the two groups of cells. One group did not respond to nerve stimulation but was spontaneously active, whereas the other responded to nerve stimulation with endplate potentials and did not discharge spontaneously. Morphological examination revealed two very similar types of longitudinal muscle cells. These results clearly show that, although morphologically similar, there are two distinct groups of muscle cells in the longitudinal muscle layer of the earthworm.

UI	MeSH Term	Description	Entries
D007546	Isoquinolines	A group of compounds with the heterocyclic ring structure of benzo(c)pyridine. The ring structure is characteristic of the group of opium alkaloids such as papaverine. (From Stedman, 25th ed)
D008839	Microelectrodes	Electrodes with an extremely small tip, used in a voltage clamp or other apparatus to stimulate or record bioelectric potentials of single cells intracellularly or extracellularly. (Dorland, 28th ed)	Electrodes, Miniaturized,Electrode, Miniaturized,Microelectrode,Miniaturized Electrode,Miniaturized Electrodes
D008856	Microscopy, Fluorescence	Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.	Fluorescence Microscopy,Immunofluorescence Microscopy,Microscopy, Immunofluorescence,Fluorescence Microscopies,Immunofluorescence Microscopies,Microscopies, Fluorescence,Microscopies, Immunofluorescence
D009132	Muscles	Contractile tissue that produces movement in animals.	Muscle Tissue,Muscle,Muscle Tissues,Tissue, Muscle,Tissues, Muscle
D009835	Oligochaeta	A class of annelid worms with few setae per segment. It includes the earthworms such as Lumbricus and Eisenia.	Earthworms,Eisenia foetida,Eisenia worm,Lumbricus,Lumbricus terrestris,Eisenia fetida,Earthworm,Eisenia fetidas,Eisenia foetidas,Eisenia worms,Lumbricus terrestri,Oligochaetas,fetida, Eisenia,foetidas, Eisenia,terrestris, Lumbricus,worm, Eisenia
D004594	Electrophysiology	The study of the generation and behavior of electrical charges in living organisms particularly the nervous system and the effects of electricity on living organisms.
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia