OBJECTIVE Hormones play a vital role in initiating and maintaining male reproductive function. The present study explores the influence and predictive ability of two reproductive hormones on semen quality among men who were partners in an infertile couple. METHODS During our cross sectional study, men were recruited from private and public hospital and laboratories for clinical evaluation of fertility status. METHODS Fresh semen samples were assessed for quality (concentration, motility and morphology) according to the 2010 World Health Organization manual and the serum levels of hormones, including follicle-stimulating hormone (FSH), Inhibin B was measured (ELISA). RESULTS We found a significant difference in the two groups regarding sperm concentration (p < 0.0001), total sperm count (p < 0.0001), total sperm count (p < 0.0001), total sperm count (p < 0.0001), total sperm count (p < 0.0001), total sperm count (. CONCLUSIONS Consistent with other studies, our results show strong association between semen quality and FSH and Inhibin B.