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Immobilization of DNA on Quartz Crystal Microbalance Sensor Modified with Self-Assembled Monolayer of Thiol Derivative. 2020

Hirobumi Shibata, and Junpei Nemoto, and Shota Shiba, and Yusuke Yamane, and Gota Kawai, and Kazuaki Hashimoto
Faculty of Engineering, Chiba Institute of Technology.

In this study, we investigated the direct detection of DNA, without pretreatment, using a quartz crystal microbalance (QCM) sensor. This sensor is modified by a self-assembled monolayer of a thiol derivative that has an amino group as the terminal functional group. Contact angle values and the attenuated total reflectance Fourier transform infrared (ATR/FT-IR) spectra of the QCM sensors after immersion into an ethanol solution of thiol derivatives clearly showed that self-assembled monolayers of the derivatives were formed on the QCM sensors. Although QCM measurements using unmodified and carboxylic group-modified sensors could not detect DNA-Na salt, the sensor modified with amino groups could detect the DNA. This system can be used for the analysis of the interaction between DNA and DNAbinding proteins.

UI MeSH Term Description Entries
D011614 Psychotherapy, Brief Any form of psychotherapy designed to produce therapeutic change within a minimal amount of time, generally not more than 20 sessions. Short-Term Psychotherapy,Brief Psychotherapy,Psychotherapy, Short-Term,Solution-Focused Brief Therapy,Brief Psychotherapies,Brief Therapies, Solution-Focused,Brief Therapy, Solution-Focused,Psychotherapies, Brief,Psychotherapies, Short-Term,Psychotherapy, Short Term,Short Term Psychotherapy,Short-Term Psychotherapies,Solution Focused Brief Therapy,Solution-Focused Brief Therapies,Therapies, Solution-Focused Brief,Therapy, Solution-Focused Brief
D004247 DNA A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine). DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D000431 Ethanol A clear, colorless liquid rapidly absorbed from the gastrointestinal tract and distributed throughout the body. It has bactericidal activity and is used often as a topical disinfectant. It is widely used as a solvent and preservative in pharmaceutical preparations as well as serving as the primary ingredient in ALCOHOLIC BEVERAGES. Alcohol, Ethyl,Absolute Alcohol,Grain Alcohol,Alcohol, Absolute,Alcohol, Grain,Ethyl Alcohol
D013438 Sulfhydryl Compounds Compounds containing the -SH radical. Mercaptan,Mercapto Compounds,Sulfhydryl Compound,Thiol,Thiols,Mercaptans,Compound, Sulfhydryl,Compounds, Mercapto,Compounds, Sulfhydryl
D017550 Spectroscopy, Fourier Transform Infrared A spectroscopic technique in which a range of wavelengths is presented simultaneously with an interferometer and the spectrum is mathematically derived from the pattern thus obtained. FTIR,Fourier Transform Infrared Spectroscopy,Spectroscopy, Infrared, Fourier Transform
D058525 Quartz Crystal Microbalance Techniques The use of a quartz crystal microbalance for measuring weights and forces in the micro- to nanogram range. It is used to study the chemical and mechanical properties of thin layers, such as polymer coatings and lipid membranes; and interactions between molecues. Electrochemical Quartz Crystal Impedance Analysis,Quartz Crystal Microbalance,Quartz Crystal Microbalance with Dissipation Monitoring,Microbalance, Quartz Crystal,Microbalances, Quartz Crystal,Quartz Crystal Microbalances

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