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In situ hybridization histochemistry and quantitative autoradiography were used to confirm the presence of cells within the bed nucleus of the stria terminalis (BNST) which express the vasopressin (VP) gene and to assess the biosynthetic capacity of these cells throughout the rostrocaudal extent of the nucleus. Brain sections from adult male Wistar rats were hybridized with a 35S-labeled 48-base oligonucleotide probe. Clusters of grains were present over cells in the BNST. Cells were parvocellular in appearance and signal over cells was determined to be specific since it was abolished by RNase pretreatment or incubation with 100-fold excess unlabeled probe. The distribution of VP-mRNA containing cells in the BNST corresponds closely to that previously reported by immunocytochemistry. No clear-cut rostral to caudal gradient was found for gene expression as measured by grains/cell. In situ hybridization techniques can provide a powerful tool to study the regulation of central VP pathways in the BNST.
M A Miller, and
R T Zoeller, and
D M Dorsa
November 1989,
Endocrinology,
M A Miller, and
R T Zoeller, and
D M Dorsa
January 1989,
Peptides,
M A Miller, and
R T Zoeller, and
D M Dorsa
May 1994,
Brain research. Developmental brain research,
M A Miller, and
R T Zoeller, and
D M Dorsa
October 1990,
Sheng li ke xue jin zhan [Progress in physiology],
M A Miller, and
R T Zoeller, and
D M Dorsa
January 1991,
Neurobiology of aging,
M A Miller, and
R T Zoeller, and
D M Dorsa
June 1986,
Brain research,
M A Miller, and
R T Zoeller, and
D M Dorsa
March 1994,
The Journal of neuroscience : the official journal of the Society for Neuroscience,
M A Miller, and
R T Zoeller, and
D M Dorsa
March 1993,
The Journal of comparative neurology,
M A Miller, and
R T Zoeller, and
D M Dorsa
January 1983,
Cell and tissue research,
M A Miller, and
R T Zoeller, and
D M Dorsa
January 1993,
Methods in enzymology,
