BIOMAT Database Logo BIOMAT Database Logo

Traceable Impedance-Based Dispensing and Cloning of Living Single Cells. 2020

Georges Muller, and David Bonzon, and Stéphanie Claudinot, and Ariane Rochat, and Philippe Renaud, and Yann Barrandon
Laboratory of Stem Cell Dynamics, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.

Single-cell cloning is essential in stem cell biology, cancer research, and biotechnology. Regulatory agencies now require an indisputable proof of clonality that current technologies do not readily provide. Here, we report a one-step cloning method using an engineered pipet combined with an impedance-based sensing tip. This technology permits the efficient and traceable isolation of living cells, stem cells, and cancer stem cells that can be individually expanded in culture and transplanted.

UI MeSH Term Description Entries
D002470 Cell Survival The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability. Cell Viability,Cell Viabilities,Survival, Cell,Viabilities, Cell,Viability, Cell
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D003412 Cricetulus A genus of the family Muridae consisting of eleven species. C. migratorius, the grey or Armenian hamster, and C. griseus, the Chinese hamster, are the two species used in biomedical research. Hamsters, Armenian,Hamsters, Chinese,Hamsters, Grey,Armenian Hamster,Armenian Hamsters,Chinese Hamster,Chinese Hamsters,Grey Hamster,Grey Hamsters,Hamster, Armenian,Hamster, Chinese,Hamster, Grey
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013234 Stem Cells Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells. Colony-Forming Units,Mother Cells,Progenitor Cells,Colony-Forming Unit,Cell, Mother,Cell, Progenitor,Cell, Stem,Cells, Mother,Cells, Progenitor,Cells, Stem,Colony Forming Unit,Colony Forming Units,Mother Cell,Progenitor Cell,Stem Cell
D016466 CHO Cells CELL LINE derived from the ovary of the Chinese hamster, Cricetulus griseus (CRICETULUS). The species is a favorite for cytogenetic studies because of its small chromosome number. The cell line has provided model systems for the study of genetic alterations in cultured mammalian cells. CHO Cell,Cell, CHO,Cells, CHO
D016475 3T3 Cells Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). Lines have been developed using several different strains of mice. Tissues are usually fibroblasts derived from mouse embryos but other types and sources have been developed as well. The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION. 3T3 Cell,Cell, 3T3,Cells, 3T3
D017097 Electric Impedance The resistance to the flow of either alternating or direct electrical current. Bioelectrical Impedance,Electric Resistance,Impedance,Ohmic Resistance,Biolectric Impedance,Electrical Impedance,Electrical Resistance,Impedance, Bioelectrical,Impedance, Biolectric,Impedance, Electric,Impedance, Electrical,Ohmic Resistances,Resistance, Electric,Resistance, Electrical,Resistance, Ohmic,Resistances, Ohmic
D045744 Cell Line, Tumor A cell line derived from cultured tumor cells. Tumor Cell Line,Cell Lines, Tumor,Line, Tumor Cell,Lines, Tumor Cell,Tumor Cell Lines

Related Publications

Georges Muller, and David Bonzon, and Stéphanie Claudinot, and Ariane Rochat, and Philippe Renaud, and Yann Barrandon
A new workflow combining magnetic cell separation and impedance-based cell dispensing for gentle, simple and reliable cloning of specific CD8+ T cells.
April 2022, SLAS technology,
Georges Muller, and David Bonzon, and Stéphanie Claudinot, and Ariane Rochat, and Philippe Renaud, and Yann Barrandon
Adhesion measurement of living cells based on electrical impedance.
January 2024, Technology and health care : official journal of the European Society for Engineering and Medicine,
Georges Muller, and David Bonzon, and Stéphanie Claudinot, and Ariane Rochat, and Philippe Renaud, and Yann Barrandon
ELECTRIC IMPEDANCE AND PERMEABILITY OF LIVING CELLS.
August 1936, Science (New York, N.Y.),
Georges Muller, and David Bonzon, and Stéphanie Claudinot, and Ariane Rochat, and Philippe Renaud, and Yann Barrandon
Ag@4ATP-coated liposomes: SERS traceable delivery vehicles for living cells.
July 2014, Nanoscale,
Georges Muller, and David Bonzon, and Stéphanie Claudinot, and Ariane Rochat, and Philippe Renaud, and Yann Barrandon
Plasmonic-Based Electrochemical Impedance Imaging of Electrical Activities in Single Cells.
July 2017, Angewandte Chemie (International ed. in English),
Georges Muller, and David Bonzon, and Stéphanie Claudinot, and Ariane Rochat, and Philippe Renaud, and Yann Barrandon
SERS-based plasmonic nanobiosensing in single living cells.
February 2009, Analytical and bioanalytical chemistry,
Georges Muller, and David Bonzon, and Stéphanie Claudinot, and Ariane Rochat, and Philippe Renaud, and Yann Barrandon
Effects of surfactant and gentle agitation on inkjet dispensing of living cells.
June 2010, Biofabrication,
Georges Muller, and David Bonzon, and Stéphanie Claudinot, and Ariane Rochat, and Philippe Renaud, and Yann Barrandon
Single cells and intracellular processes studied by a plasmonic-based electrochemical impedance microscopy.
March 2011, Nature chemistry,
Georges Muller, and David Bonzon, and Stéphanie Claudinot, and Ariane Rochat, and Philippe Renaud, and Yann Barrandon
Spectroscopic investigation of local mechanical impedance of living cells.
January 2014, PloS one,
Georges Muller, and David Bonzon, and Stéphanie Claudinot, and Ariane Rochat, and Philippe Renaud, and Yann Barrandon
Continuous differential impedance spectroscopy of single cells.
August 2010, Microfluidics and nanofluidics,
Copied contents to your clipboard!