BIOMAT Database Logo BIOMAT Database Logo

Structural characterization and immune enhancement activity of a novel polysaccharide from Moringa oleifera leaves. 2020

Chao Li, and Zhuping Dong, and Bin Zhang, and Qiang Huang, and Guang Liu, and Xiong Fu
School of Food Science and Engineering, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, National Joint Research Center for Tropical Health Foods, South China University of Technology, Guangzhou 510640, China; Overseas Expertise Introduction Center for Discipline Innovation of Food Nutrition and Human Health (111 Center), Guangzhou, China. Electronic address: felichao@scut.edu.cn.

A novel polysaccharide, designated as MOP-3, was extracted and isolated from the leaves of Moringa oleifera. Structural characterization showed that MOP-3 had a molecular weight (MW) of 4.033 × 106 Da and was composed of arabinose, glucose and galactose with a molar ratio of 47.73:1.00:57.65. Methylation and nuclear magnetic resonance (NMR) analysis revealed that the main glycosidic linkage types of MOP-3 were (1→3,6)-β-D-Galp, (1→6)-β-D-Galp, (1→5)-α-L-Araf, and T-α-L-Araf. MOP-3 containing no lipopolysaccharides showed no direct cytotoxicity to the macrophage RAW 264.7 cells within the tested concentrations. The immunomodulatory assay suggested that MOP-3 could significantly enhance pinocytic capacity and increase the secretion of reactive oxygen species (ROS), nitric oxide (NO), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) by up-regulating the corresponding mRNA expression levels in RAW 264.7 cells. Our findings demonstrated that MOP-3 could potentially be used as a functional food for the hypoimmunity therapies.

UI MeSH Term Description Entries
D011134 Polysaccharides Long chain polymeric CARBOHYDRATES composed of MONOSACCHARIDES linked by glycosidic bonds. Glycan,Glycans,Polysaccharide
D002236 Carbohydrate Conformation The characteristic 3-dimensional shape of a carbohydrate. Carbohydrate Linkage,Carbohydrate Conformations,Carbohydrate Linkages,Conformation, Carbohydrate,Conformations, Carbohydrate,Linkage, Carbohydrate,Linkages, Carbohydrate
D002470 Cell Survival The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability. Cell Viability,Cell Viabilities,Survival, Cell,Viabilities, Cell,Viability, Cell
D004305 Dose-Response Relationship, Drug The relationship between the dose of an administered drug and the response of the organism to the drug. Dose Response Relationship, Drug,Dose-Response Relationships, Drug,Drug Dose-Response Relationship,Drug Dose-Response Relationships,Relationship, Drug Dose-Response,Relationships, Drug Dose-Response
D000067996 RAW 264.7 Cells A transformed macrophage cell line isolated from ASCITES of mice infected with ABELSON MURINE LEUKEMIA VIRUS. RAW 264.7 Cell Line,264.7 Cell, RAW,264.7 Cells, RAW,Cell, RAW 264.7,Cells, RAW 264.7,RAW 264.7 Cell
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013329 Structure-Activity Relationship The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups. Relationship, Structure-Activity,Relationships, Structure-Activity,Structure Activity Relationship,Structure-Activity Relationships
D049109 Cell Proliferation All of the processes involved in increasing CELL NUMBER including CELL DIVISION. Cell Growth in Number,Cellular Proliferation,Cell Multiplication,Cell Number Growth,Growth, Cell Number,Multiplication, Cell,Number Growth, Cell,Proliferation, Cell,Proliferation, Cellular
D051379 Mice The common name for the genus Mus. Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus
D018515 Plant Leaves Expanded structures, usually green, of vascular plants, characteristically consisting of a bladelike expansion attached to a stem, and functioning as the principal organ of photosynthesis and transpiration. (American Heritage Dictionary, 2d ed) Plant Leaf,Leaf, Plant,Leave, Plant,Leaves, Plant,Plant Leave

Related Publications

Chao Li, and Zhuping Dong, and Bin Zhang, and Qiang Huang, and Guang Liu, and Xiong Fu
Structural elucidation and antioxidant activity of an arabinogalactan from the leaves of Moringa oleifera.
June 2018, International journal of biological macromolecules,
Chao Li, and Zhuping Dong, and Bin Zhang, and Qiang Huang, and Guang Liu, and Xiong Fu
In vitro digestibility and prebiotic activities of a bioactive polysaccharide from Moringa oleifera leaves.
November 2021, Journal of food biochemistry,
Chao Li, and Zhuping Dong, and Bin Zhang, and Qiang Huang, and Guang Liu, and Xiong Fu
Soluble extract from Moringa oleifera leaves with a new anticancer activity.
January 2014, PloS one,
Chao Li, and Zhuping Dong, and Bin Zhang, and Qiang Huang, and Guang Liu, and Xiong Fu
Phenolics of Moringa oleifera leaves.
January 2007, Natural product research,
Chao Li, and Zhuping Dong, and Bin Zhang, and Qiang Huang, and Guang Liu, and Xiong Fu
Extraction, purification, and activity of protease from the leaves of Moringa oleifera.
January 2018, F1000Research,
Chao Li, and Zhuping Dong, and Bin Zhang, and Qiang Huang, and Guang Liu, and Xiong Fu
Laxative Metabolites from the Leaves of Moringa oleifera.
July 2020, Journal of agricultural and food chemistry,
Chao Li, and Zhuping Dong, and Bin Zhang, and Qiang Huang, and Guang Liu, and Xiong Fu
Structural characterization of an acidic Epimedium polysaccharide and its immune-enhancement activity.
March 2016, Carbohydrate polymers,
Chao Li, and Zhuping Dong, and Bin Zhang, and Qiang Huang, and Guang Liu, and Xiong Fu
Studies on peroxidase from Moringa oleifera Lam leaves.
January 2021, Heliyon,
Chao Li, and Zhuping Dong, and Bin Zhang, and Qiang Huang, and Guang Liu, and Xiong Fu
Identification of allergen encoding sequences in a novel food ingredient from Moringa oleifera leaves.
February 2023, Food chemistry,
Chao Li, and Zhuping Dong, and Bin Zhang, and Qiang Huang, and Guang Liu, and Xiong Fu
Characterization, Large-Scale HSCCC Separation and Neuroprotective Effects of Polyphenols from Moringa oleifera Leaves.
January 2022, Molecules (Basel, Switzerland),
Copied contents to your clipboard!