The connections between the basal forebrain and the interpeduncular nucleus (IP) were studied in the rat using the anterograde transport of Phaseolus vulgaris-leucoagglutinin (PHA-L). PHA-L was injected in the septum-diagonal band complex, the preoptic area, the substantia innominata, the globus pallidus, and the ventral pallidum. In a number of cases sections of the IP were double-immunostained for PHA-L and serotonin. Only following PHA-L injections in the medial septal nucleus and the nucleus of the vertical limb of the diagonal band, were substantial terminations found throughout all subnuclei of IP. Following injections in the medial and lateral preoptic area labeling was confined to the caudal part of IP. This finding suggests that the area in the basal forebrain that contributes to these projections is smaller than has been indicated by previous retrograde tracing studies. Labeled fibers reach the IP predominantly via the medial forebrain bundle. Only a very small number of fibers reaches the ventral mesencephalon, and possibly the IP, via the stria medullaris and the fasciculus retroflexus. The highest density of terminations was seen in the apical subnucleus. The apical subnucleus contains serotonin-immunoreactive neurons, in the direct vicinity of which varicosities on the labeled fibers were seen. This finding suggests innervation of the serotonergic neurons by fibers from the medial septum and preoptic area.