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Low-intensity focused ultrasound stimulation (LIFUS) has the potential to noninvasively penetrate the intact skull and to modulate neural activity in the cortex and deep brain nuclei. The midbrain periaqueductal gray (PAG) is associated with the generation of defensive behaviors. The aim of this study was to examine whether LIFUS of the PAG induced defensive behaviors in mice. A 3.8-MHz head-mounted ultrasound transducer with a small focus size (0.5 mm × 0.5 mm) was fabricated in house to precisely stimulate the free-moving mice. The corresponding behaviors were recorded in real time. Avoidance, flight, and freezing were used to assess ultrasound-induced defensive responses. The safety of LIFUS was examined via hematoxylin and eosin (H&E) staining and Nissl staining. Ultrasound stimulation of the PAG induced multiple defensive behaviors, including location-specific passive avoidance behavior, flight, and freezing. In addition, H&E and Nissl staining verified that LIFUS did not cause any injury to the brain tissue. These findings demonstrate that LIFUS may have neuromodulatory effects on the innate defensive behaviors in mice. LIFUS may be used as a novel neuromodulatory tool for the treatment of psychological diseases associated with defensive behaviors.
Yibo Wang, and
Lili Niu, and
Wen Meng, and
Zhengrong Lin, and
Junjie Zou, and
Tianyuan Bian, and
Xiaowei Huang, and
Hui Zhou, and
Long Meng, and
Ping Xie, and
Hairong Zheng
July 2016,
The Journal of neuroscience : the official journal of the Society for Neuroscience,
Yibo Wang, and
Lili Niu, and
Wen Meng, and
Zhengrong Lin, and
Junjie Zou, and
Tianyuan Bian, and
Xiaowei Huang, and
Hui Zhou, and
Long Meng, and
Ping Xie, and
Hairong Zheng
April 2012,
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas,
Yibo Wang, and
Lili Niu, and
Wen Meng, and
Zhengrong Lin, and
Junjie Zou, and
Tianyuan Bian, and
Xiaowei Huang, and
Hui Zhou, and
Long Meng, and
Ping Xie, and
Hairong Zheng
October 2022,
IEEE transactions on bio-medical engineering,
Yibo Wang, and
Lili Niu, and
Wen Meng, and
Zhengrong Lin, and
Junjie Zou, and
Tianyuan Bian, and
Xiaowei Huang, and
Hui Zhou, and
Long Meng, and
Ping Xie, and
Hairong Zheng
January 2014,
The European journal of neuroscience,
Yibo Wang, and
Lili Niu, and
Wen Meng, and
Zhengrong Lin, and
Junjie Zou, and
Tianyuan Bian, and
Xiaowei Huang, and
Hui Zhou, and
Long Meng, and
Ping Xie, and
Hairong Zheng
August 2007,
Hormones and behavior,
Yibo Wang, and
Lili Niu, and
Wen Meng, and
Zhengrong Lin, and
Junjie Zou, and
Tianyuan Bian, and
Xiaowei Huang, and
Hui Zhou, and
Long Meng, and
Ping Xie, and
Hairong Zheng
June 2000,
Behavioural brain research,
Yibo Wang, and
Lili Niu, and
Wen Meng, and
Zhengrong Lin, and
Junjie Zou, and
Tianyuan Bian, and
Xiaowei Huang, and
Hui Zhou, and
Long Meng, and
Ping Xie, and
Hairong Zheng
November 2013,
Behavioural brain research,
Yibo Wang, and
Lili Niu, and
Wen Meng, and
Zhengrong Lin, and
Junjie Zou, and
Tianyuan Bian, and
Xiaowei Huang, and
Hui Zhou, and
Long Meng, and
Ping Xie, and
Hairong Zheng
June 1963,
Archives of neurology,
Yibo Wang, and
Lili Niu, and
Wen Meng, and
Zhengrong Lin, and
Junjie Zou, and
Tianyuan Bian, and
Xiaowei Huang, and
Hui Zhou, and
Long Meng, and
Ping Xie, and
Hairong Zheng
October 2005,
Behavioural brain research,
Yibo Wang, and
Lili Niu, and
Wen Meng, and
Zhengrong Lin, and
Junjie Zou, and
Tianyuan Bian, and
Xiaowei Huang, and
Hui Zhou, and
Long Meng, and
Ping Xie, and
Hairong Zheng
July 2008,
Life sciences,
