Biomaterial Database

Acid hydrolases in the bovine lens epithelium. 1988

T Shiono

Department of Ophthalmology, Tohoku University School of Medicine, Miyagi, Japan.

Acid hydrolases (acid phophatase, N-acetyl-beta-D-glucosaminidase, alpha-D-mannosidase, alpha-L-fucosidase, and beta-D-glucuronidase) in the bovine lens epithelium were studied biochemically. p-Nitrophenyl derivatives were used as substrate. All enzymatic activity was found to be much higher in the epithelium than in the cortex and nucleus. The properties of acid phosphatase, N-acetyl-beta-D-glucosaminidase, and alpha-D-mannosidase were also studied, yielding Km values of 0.28, 0.95, and 0.53 mM, respectively. The optimal pH of these enzymes was acidic. Among the subcellular fractions, both acid phosphatase and N-acetyl-beta-D-glucosaminidase had the highest enzymatic activities in the 20,000 g precipitate fraction, while alpha-D-mannosidase showed no difference in activity among the subcellular fractions, suggesting that alpha-D-mannosidase in the bovine lens epithelium is nonlysosomal.

UI	MeSH Term	Description	Entries
D007904	Lens Cortex, Crystalline	The portion of the crystalline lens surrounding the nucleus and bound anteriorly by the epithelium and posteriorly by the capsule. It contains lens fibers and amorphous, intercellular substance.	Cortex, Crystalline Lens,Crystalline Lens Cortex
D007907	Lens Nucleus, Crystalline	The core of the crystalline lens, surrounded by the cortex.	Crystalline Lens Nucleus,Nucleus, Crystalline Lens
D007908	Lens, Crystalline	A transparent, biconvex structure of the EYE, enclosed in a capsule and situated behind the IRIS and in front of the vitreous humor (VITREOUS BODY). It is slightly overlapped at its margin by the ciliary processes. Adaptation by the CILIARY BODY is crucial for OCULAR ACCOMMODATION.	Eye Lens,Lens, Eye,Crystalline Lens
D002417	Cattle	Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.	Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D004848	Epithelium	The layers of EPITHELIAL CELLS which cover the inner and outer surfaces of the cutaneous, mucus, and serous tissues and glands of the body.	Mesothelium,Epithelial Tissue,Mesothelial Tissue,Epithelial Tissues,Mesothelial Tissues,Tissue, Epithelial,Tissue, Mesothelial,Tissues, Epithelial,Tissues, Mesothelial
D006867	Hydrolases	Any member of the class of enzymes that catalyze the cleavage of the substrate and the addition of water to the resulting molecules, e.g., ESTERASES, glycosidases (GLYCOSIDE HYDROLASES), lipases, NUCLEOTIDASES, peptidases (PEPTIDE HYDROLASES), and phosphatases (PHOSPHORIC MONOESTER HYDROLASES). EC 3.	Hydrolase
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013347	Subcellular Fractions	Components of a cell produced by various separation techniques which, though they disrupt the delicate anatomy of a cell, preserve the structure and physiology of its functioning constituents for biochemical and ultrastructural analysis. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p163)	Fraction, Subcellular,Fractions, Subcellular,Subcellular Fraction