Densitometric identification of triglycerides separated by reversed-phase thin-layer chromatography. 1988

B Amidzhin, and B Nikolova-Damyanova
Department of Lipid Chemistry, Bulgarian Academy of Sciences, Sofia.

A method to identify triglycerides, separated by reversed-phase thin-layer chromatography on silanized Kieselguhr G layers using densitometric data only, is described. Different triglyceride mixtures, containing components with partition numbers in the range 44-56, have been scanned. A linear relationship between the partition numbers of the zones and the maximum-to-maximum distances of the corresponding peaks, relative to the peak of the zones with partition number 48, has been found. The validity of this equation has been confirmed experimentally for triglycerides with partition numbers in the range 30-46. The method allows the identification of the triglyceride components of a mixture by their partition numbers, provided only one partition number is known or can be determined independently. An example concerning sunflower oil triglycerides is presented.

UI MeSH Term Description Entries
D010938 Plant Oils Oils derived from plants or plant products. Oils, Plant,Oils, Vegetable,Plant Oil,Vegetable Oil,Vegetable Oils,Oil, Plant,Oil, Vegetable
D002849 Chromatography, Gas Fractionation of a vaporized sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix. Chromatography, Gas-Liquid,Gas Chromatography,Chromatographies, Gas,Chromatographies, Gas-Liquid,Chromatography, Gas Liquid,Gas Chromatographies,Gas-Liquid Chromatographies,Gas-Liquid Chromatography
D002855 Chromatography, Thin Layer Chromatography on thin layers of adsorbents rather than in columns. The adsorbent can be alumina, silica gel, silicates, charcoals, or cellulose. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Chromatography, Thin-Layer,Thin Layer Chromatography,Chromatographies, Thin Layer,Chromatographies, Thin-Layer,Thin Layer Chromatographies,Thin-Layer Chromatographies,Thin-Layer Chromatography
D003720 Densitometry The measurement of the density of a material by measuring the amount of light or radiation passing through (or absorbed by) the material. Densitometries
D012997 Solvents Liquids that dissolve other substances (solutes), generally solids, without any change in chemical composition, as, water containing sugar. (Grant & Hackh's Chemical Dictionary, 5th ed) Solvent
D014280 Triglycerides An ester formed from GLYCEROL and three fatty acid groups. Triacylglycerol,Triacylglycerols,Triglyceride

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