Biomaterial Database

Peptide substrates for chymosin (rennin). Kinetic studies with bovine kappa-casein-(103-108)-hexapeptide analogues. 1977

S Visser, and P J Van Rooijen, and C Schattenkerk, and K E Kerling

Kinetic parameters have been determined for the reaction between chymosin (EC 3.4.23.4) and synthetic peptide analogues of the sequence Leu-Ser-Phe-Met-Ala-Ile around the chymosin-sensitive Phe(105)-Met(106) bond of bovine kappa-casein. From the present and earlier results it is concluded that a minimum length of the molecular backbone with three amino acid units on both sides of the scissile bond is required to make the peptide a good substrate for the enzyme. In addition, hydrophobic side chains in the positions 103 and 108, and particularly the hydroxyl group of Ser-104 contribute to the effectiveness of the enzyme-substrate interactions. The substrate properties are markedly influenced by changes in the steric and/or polar character of the amino acid side chains in the positions 105 and 106.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D009842	Oligopeptides	Peptides composed of between two and twelve amino acids.	Oligopeptide
D012085	Chymosin	The predominant milk-clotting enzyme from the true stomach or abomasum of the suckling calf. It is secreted as an inactive precursor called prorennin and converted in the acid environment of the stomach to the active enzyme. EC 3.4.23.4.	Rennin,Chymosin A,Chymosin C
D002364	Caseins	A mixture of related phosphoproteins occurring in milk and cheese. The group is characterized as one of the most nutritive milk proteins, containing all of the common amino acids and rich in the essential ones.	alpha-Casein,gamma-Casein,AD beta-Casein,Acetylated, Dephosphorylated beta-Casein,Casein,Casein A,K-Casein,Sodium Caseinate,alpha(S1)-Casein,alpha(S1)-Casein A,alpha(S1)-Casein B,alpha(S1)-Casein C,alpha(S2)-Casein,alpha-Caseins,beta-Casein,beta-Caseins,epsilon-Casein,gamma-Caseins,kappa-Casein,kappa-Caseins,AD beta Casein,Caseinate, Sodium,K Casein,alpha Casein,alpha Caseins,beta Casein,beta Caseins,beta-Casein Acetylated, Dephosphorylated,beta-Casein, AD,epsilon Casein,gamma Casein,gamma Caseins,kappa Casein,kappa Caseins
D013329	Structure-Activity Relationship	The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.	Relationship, Structure-Activity,Relationships, Structure-Activity,Structure Activity Relationship,Structure-Activity Relationships