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Limited proteolysis of bovine colostral IgG1 by trypsin caused loss of specific antibody activity but column chromatography showed that relatively little cleavage into fragements had occurred. Polyacrlamide-agarose SDS electrophoresis of the 2-mercaptoethanol-treated digest revealed, however, that extensive cleavage of light chains had occurred even though most of the material before reduction had a mol. wt close to that of undigested IgG1. Although a Fab-type fragment was detected in the digest by immunoelectrophoresis it appeared to be only a minor component. Chymotrypsin had little effect upon either the structure or antibody activity of IgG1. These findings may explain the effect of trypsin and chymotrypsin on the bactericidal activity of colostral antibodies.
J H Brock, and
F R Arzabe, and
F Ortega, and
A Piñeiro
November 1972,
European journal of biochemistry,
J H Brock, and
F R Arzabe, and
F Ortega, and
A Piñeiro
June 1970,
Biochemistry,
J H Brock, and
F R Arzabe, and
F Ortega, and
A Piñeiro
August 1980,
Biochemistry,
J H Brock, and
F R Arzabe, and
F Ortega, and
A Piñeiro
March 2023,
Food research international (Ottawa, Ont.),
J H Brock, and
F R Arzabe, and
F Ortega, and
A Piñeiro
August 1981,
Biochimica et biophysica acta,
J H Brock, and
F R Arzabe, and
F Ortega, and
A Piñeiro
July 1978,
Journal of immunology (Baltimore, Md. : 1950),
J H Brock, and
F R Arzabe, and
F Ortega, and
A Piñeiro
August 1988,
FEBS letters,
J H Brock, and
F R Arzabe, and
F Ortega, and
A Piñeiro
October 1968,
The Journal of biological chemistry,
J H Brock, and
F R Arzabe, and
F Ortega, and
A Piñeiro
January 1978,
Annales de recherches veterinaires. Annals of veterinary research,
J H Brock, and
F R Arzabe, and
F Ortega, and
A Piñeiro
July 1975,
FEBS letters,
