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Cultured mammalian cells extend the time of survival of Treponema pallidum (Nichols strain). Various parameters that have been previously shown to enhance treponemal survival in vitro were examined for influences on the interaction of T. pallidum with cultured cells. With cells derived from normal rabbit testes, the time of retention of treponemal virulence was extended in an atmosphere containing reduced concentrations of oxygen. Glutathione and cysteine, when added to the basal tissue culture medium, prolonged treponemal survival. In an assessment of various tissue culture medium supplements, normal rabbit serum was equivalent to fetal bovine serum and superior to bovine serum albumin fraction V (BSA), fatty acid-poor BSA, and lipid-pooed for TRK-2, HSE, NRK, and C6 cells. Dithiotreitol, as an additional reducing agent, sharply enhanced treponemal survival. With SF1Ep NBL-11 cells and basal tissue culture medium containing glutathione, cysteine, and dithiothreitol, in an atmosphere of approximately 3% oxygen, T. pallidum was maintained without detectable decreases in the number of virulent organisms for 6 days.
T J Fitzgerald, and
R C Johnson, and
J A Sykes, and
J N Miller
January 1957,
Comptes rendus des seances de la Societe de biologie et de ses filiales,
T J Fitzgerald, and
R C Johnson, and
J A Sykes, and
J N Miller
May 1970,
Klinische Wochenschrift,
T J Fitzgerald, and
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J A Sykes, and
J N Miller
June 1979,
Infection and immunity,
T J Fitzgerald, and
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J N Miller
January 1966,
Dermatologica,
T J Fitzgerald, and
R C Johnson, and
J A Sykes, and
J N Miller
August 1983,
Infection and immunity,
T J Fitzgerald, and
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J N Miller
January 1988,
Infection and immunity,
T J Fitzgerald, and
R C Johnson, and
J A Sykes, and
J N Miller
May 1978,
Nihon saikingaku zasshi. Japanese journal of bacteriology,
T J Fitzgerald, and
R C Johnson, and
J A Sykes, and
J N Miller
March 1969,
Der Hautarzt; Zeitschrift fur Dermatologie, Venerologie, und verwandte Gebiete,
T J Fitzgerald, and
R C Johnson, and
J A Sykes, and
J N Miller
December 2007,
Infection and immunity,
T J Fitzgerald, and
R C Johnson, and
J A Sykes, and
J N Miller
August 1952,
Comptes rendus des seances de la Societe de biologie et de ses filiales,
