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Three peaks of proteinases were observed with hemoglobin, bovine serum albumin and casein as substrates at the pH of 3.5, 6.5 and 8.5, in prenatal human cerebral cortex. Cathepsin D (EC 3.4.23.5) was the most prominent, with hemoglobin as the preferred substrate. The enzyme was partially purified by Concanavalin A - Sepharose affinity chromatography and the nature of the active site was assessed with proteinase inhibitors. Inhibitor studies showed that similar to pepstatin A, benzethonium chloride was also strongly inhibitory to the enzyme. The distribution of cathepsin D, a neuronal marker, and 2',3'-cyclic nucleotide 3'-phosphohydrolase (EC 3.1.4.37), a oligodendroglial marker in foetal brain regions with increasing gestation revealed that neurogenesis and gliogenesis occur concomitantly from earlier periods of gestation. Glial marker acquisition was particularly high in medulla and in spinal cord between 20 and 25 weeks of gestation.
R G Mahajan, and
S Mandal, and
K L Mukherjee
April 1973,
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R G Mahajan, and
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K L Mukherjee
June 1974,
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R G Mahajan, and
S Mandal, and
K L Mukherjee
August 1968,
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R G Mahajan, and
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K L Mukherjee
October 1977,
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R G Mahajan, and
S Mandal, and
K L Mukherjee
October 1969,
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R G Mahajan, and
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K L Mukherjee
February 1972,
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R G Mahajan, and
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K L Mukherjee
October 1980,
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R G Mahajan, and
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K L Mukherjee
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R G Mahajan, and
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K L Mukherjee
September 1980,
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R G Mahajan, and
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K L Mukherjee
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