Biomaterial Database

The determination of the amino acid sequence in the fast atom bombardment mass spectra of dipeptides. 1988

W Kulik, and W Heerma

Laboratory for Analytical Chemistry, University of Utrecht, The Netherlands.

The positive ion fast atom bombardment mass spectra of dipeptides all exhibit the MH+ ion as the most abundant one. Direct identification of the N-terminal amino acid in the mass spectrum is not possible because of the absence of its acylium ion. Fortunately the identification of the C-terminus is often possible because of the occurrence of the protonated C-terminal amino acid as a result of MH+ ion fragmentation. In all cases, including those where the abundance of this C-terminal ion is too low for identification, the metastable ion spectrum of the MH+ ion provides sufficient information for an unequivocal sequence determination.

UI	MeSH Term	Description	Entries
D004151	Dipeptides	Peptides composed of two amino acid units.	Dipeptide
D000595	Amino Acid Sequence	The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.	Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D013058	Mass Spectrometry	An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.	Mass Spectroscopy,Spectrometry, Mass,Spectroscopy, Mass,Spectrum Analysis, Mass,Analysis, Mass Spectrum,Mass Spectrum Analysis,Analyses, Mass Spectrum,Mass Spectrum Analyses,Spectrum Analyses, Mass