BACKGROUND Antioxidants are chemical compounds that can donate one or more electrons to free radicals to prevent degenerative diseases. The crown of pineapple (Ananas comosus (L.) Merr.) contains bromelain, that is, a proteolytic enzyme, which can act as an antioxidant. OBJECTIVE The aim of this study was to determine the antioxidant activity of crude bromelain from pineapple crowns that were collected from Subang district, West Java, Indonesia. METHODS Antioxidant activity was determined by the method of 2,2-diphenyl-1-picrylhydrazyl (DPPH) with vitamin C as a standard and measured with visible spectrophotometer. RESULTS The pineapple crown was produced 0.26% of dried crude bromelain with total protein content 44.10% and IC50 value 3624 μg/mL of crude bromelain, which was equivalent to 1590.18 μg/mL of total protein. CONCLUSIONS Crude bromelain has medium antioxidant activity.