-transparent.png){kind=logo}
Fluorogenic Bifunctional trans-Cyclooctenes as Efficient Tools for Investigating Click-to-Release Kinetics. 2020
Mark A R de Geus, and
Elmer Maurits, and
Alexi J C Sarris, and
Thomas Hansen, and
Max S Kloet, and
Kiki Kamphorst, and
Wolter Ten Hoeve, and
Marc S Robillard, and
Andrea Pannwitz, and
Sylvestre A Bonnet, and
Jeroen D C Codée, and
Dmitri V Filippov, and
Herman S Overkleeft, and
Sander I van Kasteren
Leiden Institute of Chemistry and The Institute for Chemical Immunology, Leiden University, Einsteinweg 55, 2333 CC, Leiden, The Netherlands.
The inverse electron demand Diels-Alder pyridazine elimination reaction between tetrazines and allylic substituted trans-cyclooctenes (TCOs) is a key player in bioorthogonal bond cleavage reactions. Determining the rate of elimination of alkylamine substrates has so far proven difficult. Here, we report a fluorogenic tool consisting of a TCO-linked EDANS fluorophore and a DABCYL quencher for accurate determination of both the click and release rate constants for any tetrazine at physiologically relevant concentrations.
| UI | MeSH Term | Description | Entries |
|---|
Related Publications
Mark A R de Geus, and
Elmer Maurits, and
Alexi J C Sarris, and
Thomas Hansen, and
Max S Kloet, and
Kiki Kamphorst, and
Wolter Ten Hoeve, and
Marc S Robillard, and
Andrea Pannwitz, and
Sylvestre A Bonnet, and
Jeroen D C Codée, and
Dmitri V Filippov, and
Herman S Overkleeft, and
Sander I van Kasteren
January 2023,
Chemistry (Weinheim an der Bergstrasse, Germany),
Mark A R de Geus, and
Elmer Maurits, and
Alexi J C Sarris, and
Thomas Hansen, and
Max S Kloet, and
Kiki Kamphorst, and
Wolter Ten Hoeve, and
Marc S Robillard, and
Andrea Pannwitz, and
Sylvestre A Bonnet, and
Jeroen D C Codée, and
Dmitri V Filippov, and
Herman S Overkleeft, and
Sander I van Kasteren
April 2020,
Chembiochem : a European journal of chemical biology,
Mark A R de Geus, and
Elmer Maurits, and
Alexi J C Sarris, and
Thomas Hansen, and
Max S Kloet, and
Kiki Kamphorst, and
Wolter Ten Hoeve, and
Marc S Robillard, and
Andrea Pannwitz, and
Sylvestre A Bonnet, and
Jeroen D C Codée, and
Dmitri V Filippov, and
Herman S Overkleeft, and
Sander I van Kasteren
July 2020,
Chemical communications (Cambridge, England),
Mark A R de Geus, and
Elmer Maurits, and
Alexi J C Sarris, and
Thomas Hansen, and
Max S Kloet, and
Kiki Kamphorst, and
Wolter Ten Hoeve, and
Marc S Robillard, and
Andrea Pannwitz, and
Sylvestre A Bonnet, and
Jeroen D C Codée, and
Dmitri V Filippov, and
Herman S Overkleeft, and
Sander I van Kasteren
October 2018,
Angewandte Chemie (International ed. in English),
Mark A R de Geus, and
Elmer Maurits, and
Alexi J C Sarris, and
Thomas Hansen, and
Max S Kloet, and
Kiki Kamphorst, and
Wolter Ten Hoeve, and
Marc S Robillard, and
Andrea Pannwitz, and
Sylvestre A Bonnet, and
Jeroen D C Codée, and
Dmitri V Filippov, and
Herman S Overkleeft, and
Sander I van Kasteren
August 2018,
Angewandte Chemie (International ed. in English),
Mark A R de Geus, and
Elmer Maurits, and
Alexi J C Sarris, and
Thomas Hansen, and
Max S Kloet, and
Kiki Kamphorst, and
Wolter Ten Hoeve, and
Marc S Robillard, and
Andrea Pannwitz, and
Sylvestre A Bonnet, and
Jeroen D C Codée, and
Dmitri V Filippov, and
Herman S Overkleeft, and
Sander I van Kasteren
January 2016,
Monatshefte fur chemie,
Mark A R de Geus, and
Elmer Maurits, and
Alexi J C Sarris, and
Thomas Hansen, and
Max S Kloet, and
Kiki Kamphorst, and
Wolter Ten Hoeve, and
Marc S Robillard, and
Andrea Pannwitz, and
Sylvestre A Bonnet, and
Jeroen D C Codée, and
Dmitri V Filippov, and
Herman S Overkleeft, and
Sander I van Kasteren
December 2023,
Chemistry (Weinheim an der Bergstrasse, Germany),
Mark A R de Geus, and
Elmer Maurits, and
Alexi J C Sarris, and
Thomas Hansen, and
Max S Kloet, and
Kiki Kamphorst, and
Wolter Ten Hoeve, and
Marc S Robillard, and
Andrea Pannwitz, and
Sylvestre A Bonnet, and
Jeroen D C Codée, and
Dmitri V Filippov, and
Herman S Overkleeft, and
Sander I van Kasteren
January 1992,
Clinical neuropharmacology,
Mark A R de Geus, and
Elmer Maurits, and
Alexi J C Sarris, and
Thomas Hansen, and
Max S Kloet, and
Kiki Kamphorst, and
Wolter Ten Hoeve, and
Marc S Robillard, and
Andrea Pannwitz, and
Sylvestre A Bonnet, and
Jeroen D C Codée, and
Dmitri V Filippov, and
Herman S Overkleeft, and
Sander I van Kasteren
August 2023,
Chemistry (Weinheim an der Bergstrasse, Germany),
Mark A R de Geus, and
Elmer Maurits, and
Alexi J C Sarris, and
Thomas Hansen, and
Max S Kloet, and
Kiki Kamphorst, and
Wolter Ten Hoeve, and
Marc S Robillard, and
Andrea Pannwitz, and
Sylvestre A Bonnet, and
Jeroen D C Codée, and
Dmitri V Filippov, and
Herman S Overkleeft, and
Sander I van Kasteren
January 2015,
Invertebrate reproduction & development,
