BIOMAT Database Logo BIOMAT Database Logo

[Synthesis of tritium-labelled prostaglandin E1 and its binding to human platelets]. 1988

V P Shevchenko, and I Iu Nagaev, and P V Vrzheshch, and D E Ershov, and S V Zaĭtsev

A method has been developed that makes it possible to obtain [5,6-3H2]PGE1 with a yield of 35% and a molar radioactivity of 1.7-1.8 TBq/mmol. The binding of [5,6-3H2]PGE1 to native platelets proved to be specific, saturating and reversible. It is characterized by low values (approximately 10(-9) M) of dissociation constants for high-affinity sites, correlates with the inhibition of ADP-induced aggregation of platelets and can be considered as receptor binding. Specific binding of 10 +/- 2 molecules of PGE1 with one platelet was found to cause 50% inhibition of the ADP-induced aggregation.

UI MeSH Term Description Entries
D007553 Isotope Labeling Techniques for labeling a substance with a stable or radioactive isotope. It is not used for articles involving labeled substances unless the methods of labeling are substantively discussed. Tracers that may be labeled include chemical substances, cells, or microorganisms. Isotope Labeling, Stable,Isotope-Coded Affinity Tagging,Isotopically-Coded Affinity Tagging,Affinity Tagging, Isotope-Coded,Affinity Tagging, Isotopically-Coded,Isotope Coded Affinity Tagging,Labeling, Isotope,Labeling, Stable Isotope,Stable Isotope Labeling,Tagging, Isotope-Coded Affinity,Tagging, Isotopically-Coded Affinity
D007700 Kinetics The rate dynamics in chemical or physical systems.
D008024 Ligands A molecule that binds to another molecule, used especially to refer to a small molecule that binds specifically to a larger molecule, e.g., an antigen binding to an antibody, a hormone or neurotransmitter binding to a receptor, or a substrate or allosteric effector binding to an enzyme. Ligands are also molecules that donate or accept a pair of electrons to form a coordinate covalent bond with the central metal atom of a coordination complex. (From Dorland, 27th ed) Ligand
D010974 Platelet Aggregation The attachment of PLATELETS to one another. This clumping together can be induced by a number of agents (e.g., THROMBIN; COLLAGEN) and is part of the mechanism leading to the formation of a THROMBUS. Aggregation, Platelet
D011869 Radioligand Assay Quantitative determination of receptor (binding) proteins in body fluids or tissue using radioactively labeled binding reagents (e.g., antibodies, intracellular receptors, plasma binders). Protein-Binding Radioassay,Radioreceptor Assay,Assay, Radioligand,Assay, Radioreceptor,Assays, Radioligand,Assays, Radioreceptor,Protein Binding Radioassay,Protein-Binding Radioassays,Radioassay, Protein-Binding,Radioassays, Protein-Binding,Radioligand Assays,Radioreceptor Assays
D001792 Blood Platelets Non-nucleated disk-shaped cells formed in the megakaryocyte and found in the blood of all mammals. They are mainly involved in blood coagulation. Platelets,Thrombocytes,Blood Platelet,Platelet,Platelet, Blood,Platelets, Blood,Thrombocyte
D002621 Chemistry A basic science concerned with the composition, structure, and properties of matter; and the reactions that occur between substances and the associated energy exchange.
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000244 Adenosine Diphosphate Adenosine 5'-(trihydrogen diphosphate). An adenine nucleotide containing two phosphate groups esterified to the sugar moiety at the 5'-position. ADP,Adenosine Pyrophosphate,Magnesium ADP,MgADP,Adenosine 5'-Pyrophosphate,5'-Pyrophosphate, Adenosine,ADP, Magnesium,Adenosine 5' Pyrophosphate,Diphosphate, Adenosine,Pyrophosphate, Adenosine
D000527 Alprostadil A potent vasodilator agent that increases peripheral blood flow. PGE1,Prostaglandin E1,Caverject,Edex,Lipo-PGE1,Minprog,Muse,PGE1alpha,Prostaglandin E1alpha,Prostavasin,Prostin VR,Prostine VR,Sugiran,Vasaprostan,Viridal,Lipo PGE1

Related Publications

V P Shevchenko, and I Iu Nagaev, and P V Vrzheshch, and D E Ershov, and S V Zaĭtsev
Prostaglandin E1 and platelets.
March 1973, Biomedicine / [publiee pour l'A.A.I.C.I.G.],
V P Shevchenko, and I Iu Nagaev, and P V Vrzheshch, and D E Ershov, and S V Zaĭtsev
The absorption of tritium-labelled prostaglandin E1 from the vagina of non-pregnant women.
January 1968, Acta obstetricia et gynecologica Scandinavica,
V P Shevchenko, and I Iu Nagaev, and P V Vrzheshch, and D E Ershov, and S V Zaĭtsev
Effect of prostaglandin E1 on deposition of autologous labelled platelets onto human atherosclerotic lesions in vivo.
April 1987, Postgraduate medical journal,
V P Shevchenko, and I Iu Nagaev, and P V Vrzheshch, and D E Ershov, and S V Zaĭtsev
Cyclic AMP induces synthesis of prostaglandin E1 in platelets.
September 1983, Biochimica et biophysica acta,
V P Shevchenko, and I Iu Nagaev, and P V Vrzheshch, and D E Ershov, and S V Zaĭtsev
Binding of prostaglandin E1 to human erythrocyte membrane.
February 1985, Biochimica et biophysica acta,
V P Shevchenko, and I Iu Nagaev, and P V Vrzheshch, and D E Ershov, and S V Zaĭtsev
Tritium-labelled alkaloids: Synthesis and applications.
February 2017, Journal of labelled compounds & radiopharmaceuticals,
V P Shevchenko, and I Iu Nagaev, and P V Vrzheshch, and D E Ershov, and S V Zaĭtsev
6-Keto-prostaglandin E1 inhibits the aggregation of human platelets.
December 1979, European journal of pharmacology,
V P Shevchenko, and I Iu Nagaev, and P V Vrzheshch, and D E Ershov, and S V Zaĭtsev
Prostaglandin E1 specific binding in human myometrium.
November 1975, Biology of reproduction,
V P Shevchenko, and I Iu Nagaev, and P V Vrzheshch, and D E Ershov, and S V Zaĭtsev
Synthesis and characterization of tritium-labelled substances.
July 2018, Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine,
V P Shevchenko, and I Iu Nagaev, and P V Vrzheshch, and D E Ershov, and S V Zaĭtsev
Synthesis of tritium-labelled tetrahydrocannabinol and cannabidiol.
January 1973, Acta chemica Scandinavica,
Copied contents to your clipboard!