Identification and characterization of microRNAs in American cockroach (Periplaneta americana). 2020

Qiao Yang, and Zheng Bao, and Mingyu Yang, and Yongmei Shen, and Xiuyue Zhang, and Bisong Yue, and Yang Meng, and Zhenxin Fan
Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, Sichuan, People's Republic of China.

The American cockroach (Periplaneta americana), which is one of the most ancient insects in the world, is characterized by incomplete metamorphosis. So far, no one has studied the microRNA profile of P. americana and the changes in the expression of microRNAs on different developmental stages. In this study, we sequenced the small RNAs (sRNA) of four samples at four post-embryonic developmental stages. A total of 35 conserved known mature miRNAs and 134 novel mature miRNAs were identified. After differential expression analysis, we noticed there were more miRNA expressed differentially during early post-embryonic stages. We also found about one-third of known miRNAs were significantly dynamic. After a cross-species comparative analysis, we identified two miRNAs (miR-1000 and miR-932) that may be important for morphogenesis, olfactory, learning, and memory. In addition, unlike hetero-metabolous insects, miRNAs still played an important role in late post-embryonic stages in holo-metabolous insects. In conclusion, our results underline the significance of miRNA expression in post-embryonic developmental stages of the American cockroach and push forward the understanding of gene expression modulation by miRNAs in cockroach. This would also contribute to the study of gene expression regulation by miRNAs during metamorphosis development.

UI MeSH Term Description Entries
D007814 Larva Wormlike or grublike stage, following the egg in the life cycle of insects, worms, and other metamorphosing animals. Maggots,Tadpoles,Larvae,Maggot,Tadpole
D008297 Male Males
D008675 Metamorphosis, Biological Profound physical changes during maturation of living organisms from the immature forms to the adult forms, such as from TADPOLES to frogs; caterpillars to BUTTERFLIES. Biological Metamorphosis,Biological Metamorphoses,Metamorphoses, Biological
D010526 Periplaneta A genus in the family Blattidae containing several species, the most common being P. americana, the American cockroach. Periplaneta americana,American Cockroach,American Cockroaches,American Cockroache,American Cockroachs,Cockroach, American,Cockroache, American,Cockroaches, American,Cockroachs, American,Periplaneta americanas,Periplanetas,americana, Periplaneta
D003071 Cognition Intellectual or mental process whereby an organism obtains knowledge. Cognitive Function,Cognitions,Cognitive Functions,Function, Cognitive,Functions, Cognitive
D005260 Female Females
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D017423 Sequence Analysis, RNA A multistage process that includes cloning, physical mapping, subcloning, sequencing, and information analysis of an RNA SEQUENCE. RNA Sequence Analysis,Sequence Determination, RNA,Analysis, RNA Sequence,Determination, RNA Sequence,Determinations, RNA Sequence,RNA Sequence Determination,RNA Sequence Determinations,RNA Sequencing,Sequence Determinations, RNA,Analyses, RNA Sequence,RNA Sequence Analyses,Sequence Analyses, RNA,Sequencing, RNA
D018507 Gene Expression Regulation, Developmental Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action during the developmental stages of an organism. Developmental Gene Expression Regulation,Embryologic Gene Expression Regulation,Gene Expression Regulation, Embryologic,Regulation of Gene Expression, Developmental,Regulation of Gene Expression, Embryologic,Regulation, Gene Expression, Developmental,Regulation, Gene Expression, Embryologic
D020869 Gene Expression Profiling The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell. Gene Expression Analysis,Gene Expression Pattern Analysis,Transcript Expression Analysis,Transcriptome Profiling,Transcriptomics,mRNA Differential Display,Gene Expression Monitoring,Transcriptome Analysis,Analyses, Gene Expression,Analyses, Transcript Expression,Analyses, Transcriptome,Analysis, Gene Expression,Analysis, Transcript Expression,Analysis, Transcriptome,Differential Display, mRNA,Differential Displays, mRNA,Expression Analyses, Gene,Expression Analysis, Gene,Gene Expression Analyses,Gene Expression Monitorings,Gene Expression Profilings,Monitoring, Gene Expression,Monitorings, Gene Expression,Profiling, Gene Expression,Profiling, Transcriptome,Profilings, Gene Expression,Profilings, Transcriptome,Transcript Expression Analyses,Transcriptome Analyses,Transcriptome Profilings,mRNA Differential Displays

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