Biomaterial Database

Identification of anti-Sm and anti-RNP autoantibodies by immunoblotting and immunoassay. 1988

N Zurgil, and S Shafrir, and H Slor, and Y Shoenfeld

Department of Human Genetics, Sackler Faculty of Medicine, Tel Aviv University, Israel.

UI	MeSH Term	Description	Entries
D007122	Immunoelectrophoresis	A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
D008947	Mixed Connective Tissue Disease	A syndrome with overlapping clinical features of systemic lupus erythematosus, scleroderma, polymyositis, and Raynaud's phenomenon. The disease is differentially characterized by high serum titers of antibodies to ribonuclease-sensitive extractable (saline soluble) nuclear antigen and a "speckled" epidermal nuclear staining pattern on direct immunofluorescence.	Connective Tissue Disease, Mixed,Sharp Syndrome,MCTD,Syndrome, Sharp
D004797	Enzyme-Linked Immunosorbent Assay	An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.	ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001323	Autoantibodies	Antibodies that react with self-antigens (AUTOANTIGENS) of the organism that produced them.	Autoantibody
D001324	Autoantigens	Endogenous tissue constituents with the ability to interact with AUTOANTIBODIES and cause an immune response.	Autoantigen,Autologous Antigen,Autologous Antigens,Self-Antigen,Self-Antigens,Antigen, Autologous,Antigens, Autologous,Self Antigen,Self Antigens
D012261	Ribonucleoproteins	Complexes of RNA-binding proteins with ribonucleic acids (RNA).	Ribonucleoprotein
D015153	Blotting, Western	Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.	Immunoblotting, Western,Western Blotting,Western Immunoblotting,Blot, Western,Immunoblot, Western,Western Blot,Western Immunoblot,Blots, Western,Blottings, Western,Immunoblots, Western,Immunoblottings, Western,Western Blots,Western Blottings,Western Immunoblots,Western Immunoblottings
D017411	Ribonucleoproteins, Small Nuclear	Highly conserved nuclear RNA-protein complexes that function in RNA processing in the nucleus, including pre-mRNA splicing and pre-mRNA 3'-end processing in the nucleoplasm, and pre-rRNA processing in the nucleolus (see RIBONUCLEOPROTEINS, SMALL NUCLEOLAR).	Small Nuclear Ribonucleoproteins,snRNP,Small Nuclear RNP,Small Nuclear Ribonucleoprotein,Small Nuclear Ribonucleoprotein Particle,Nuclear RNP, Small,Nuclear Ribonucleoprotein, Small,Nuclear Ribonucleoproteins, Small,RNP, Small Nuclear,Ribonucleoprotein, Small Nuclear
D055517	snRNP Core Proteins	The protein components that constitute the common core of small nuclear ribonucleoprotein particles. These proteins are commonly referred as Sm nuclear antigens due to their antigenic nature.	Sm Nuclear Antigens,SNRPN,SNRPN Protein,Sm Core Proteins,SmD Antigen,SmD Autoantigen,Small Nuclear Ribonucleoprotein B,Small Nuclear Ribonucleoprotein Core Proteins,Small Nuclear Ribonucleoprotein D1 Polypeptide 16kDa,Small Nuclear Ribonucleoprotein D2 Polypeptide 16.5kDa,Small Nuclear Ribonucleoprotein D3 Polypeptide 18kDa,Small Nuclear Ribonucleoprotein Polypeptide B',Small Nuclear Ribonucleoprotein Polypeptide B'',Small Nuclear Ribonucleoprotein Polypeptide B2,Small Nuclear Ribonucleoprotein Polypeptide E,Small Nuclear Ribonucleoprotein Polypeptide F,Small Nuclear Ribonucleoprotein Sm D1,Small Nuclear Ribonucleoprotein Sm D2,Small Nuclear Ribonucleoprotein-Associated Protein B,Small Nuclear Ribonucleoprotein-Associated Protein B1,Small Nuclear Ribonucleoprotein-Associated Protein B2,Small Nuclear Ribonucleoprotein-Associated Protein D1,Small Nuclear Ribonucleoprotein-Associated Protein D2,Small Nuclear Ribonucleoprotein-Associated Protein D3,Small Nuclear Ribonucleoprotein-Associated Protein E,Small Nuclear Ribonucleoprotein-Associated Protein F,Small Nuclear Ribonucleoprotein-Associated Protein N,snRNP Core Protein D1,Antigen, SmD,Antigens, Sm Nuclear,Autoantigen, SmD,Core Proteins, Sm,Core Proteins, snRNP,Nuclear Antigens, Sm,Small Nuclear Ribonucleoprotein Associated Protein B,Small Nuclear Ribonucleoprotein Associated Protein B1,Small Nuclear Ribonucleoprotein Associated Protein B2,Small Nuclear Ribonucleoprotein Associated Protein D1,Small Nuclear Ribonucleoprotein Associated Protein D2,Small Nuclear Ribonucleoprotein Associated Protein D3,Small Nuclear Ribonucleoprotein Associated Protein E,Small Nuclear Ribonucleoprotein Associated Protein F,Small Nuclear Ribonucleoprotein Associated Protein N